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PDBsum entry 4buj
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Contents |
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903 a.a.
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1367 a.a.
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392 a.a.
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351 a.a.
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References listed in PDB file
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Key reference
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Title
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The yeast ski complex: crystal structure and RNA channeling to the exosome complex.
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Authors
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F.Halbach,
P.Reichelt,
M.Rode,
E.Conti.
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Ref.
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Cell, 2013,
154,
814-826.
[DOI no: ]
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PubMed id
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Abstract
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The Ski complex is a conserved multiprotein assembly required for the
cytoplasmic functions of the exosome, including RNA turnover, surveillance, and
interference. Ski2, Ski3, and Ski8 assemble in a tetramer with 1:1:2
stoichiometry. The crystal structure of an S. cerevisiae 370 kDa core complex
shows that Ski3 forms an array of 33 TPR motifs organized in N-terminal and
C-terminal arms. The C-terminal arm of Ski3 and the two Ski8 subunits position
the helicase core of Ski2 centrally within the complex, enhancing RNA binding.
The Ski3 N-terminal arm and the Ski2 insertion domain allosterically modulate
the ATPase and helicase activities of the complex. Biochemical data suggest that
the Ski complex can thread RNAs directly to the exosome, coupling the helicase
and the exoribonuclease through a continuous RNA channel. Finally, we identify a
Ski8-binding motif common to Ski3 and Spo11, rationalizing the moonlighting
properties of Ski8 in mRNA decay and meiosis.
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