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PDBsum entry 4bqb
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Cell adhesion
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PDB id
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4bqb
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PDB id:
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Cell adhesion
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Title:
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Crystal structure of the fn5 and fn6 domains of neo1, form 2
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Structure:
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Neogenin. Chain: a, b, c, d. Fragment: fn-type iii domains 5 and 6, residues 883-1133. Engineered: yes. Other_details: n-linked glycosylation at n940
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Source:
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Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293t.
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Resolution:
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2.70Å
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R-factor:
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0.201
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R-free:
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0.223
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Authors:
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C.H.Bell,E.Healey,S.Van Erp,B.Bishop,C.Tang,R.J.C.Gilbert, A.R.Aricescu,R.J.Pasterkamp,C.Siebold
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Key ref:
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C.H.Bell
et al.
(2013).
Structure of the repulsive guidance molecule (RGM)-neogenin signaling hub.
Science,
341,
77-80.
PubMed id:
DOI:
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Date:
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30-May-13
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Release date:
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12-Jun-13
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PROCHECK
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Headers
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References
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P97798
(NEO1_MOUSE) -
Neogenin from Mus musculus
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Seq:
Struc:
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1492 a.a.
195 a.a.
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Key: |
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Secondary structure |
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CATH domain |
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DOI no:
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Science
341:77-80
(2013)
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PubMed id:
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Structure of the repulsive guidance molecule (RGM)-neogenin signaling hub.
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C.H.Bell,
E.Healey,
S.van Erp,
B.Bishop,
C.Tang,
R.J.Gilbert,
A.R.Aricescu,
R.J.Pasterkamp,
C.Siebold.
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ABSTRACT
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Repulsive guidance molecule family members (RGMs) control fundamental and
diverse cellular processes, including motility and adhesion, immune cell
regulation, and systemic iron metabolism. However, it is not known how RGMs
initiate signaling through their common cell-surface receptor, neogenin (NEO1).
Here, we present crystal structures of the NEO1 RGM-binding region and its
complex with human RGMB (also called dragon). The RGMB structure reveals a
previously unknown protein fold and a functionally important autocatalytic
cleavage mechanism and provides a framework to explain numerous disease-linked
mutations in RGMs. In the complex, two RGMB ectodomains conformationally
stabilize the juxtamembrane regions of two NEO1 receptors in a pH-dependent
manner. We demonstrate that all RGM-NEO1 complexes share this architecture,
which therefore represents the core of multiple signaling pathways.
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