PDBsum entry 4ayd

Immune system

PDB id: 4ayd

Contents

Protein chains

123 a.a.

117 a.a.

238 a.a.

Ligands

EDO ×4

Waters ×826

PDB id:

4ayd

Name:

Immune system

Title:

Structure of a complex between ccps 6 and 7 of human complement factor h and neisseria meningitidis fhbp variant 1 r106a mutant

Structure:

Complement factor h. Chain: a, b, e. Fragment: ccps 6 and 7, residues 321-443. Synonym: h factor 1. Engineered: yes. Factor h binding protein. Chain: c, d, f. Fragment: residues 73-320. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: his402 polymorphism. Neisseria meningitidis. Organism_taxid: 122586. Strain: mc58.

Resolution:

2.40Å R-factor: 0.204 R-free: 0.231

Authors:

S.Johnson,L.Tan,S.Van Der Veen,J.Caesar,E.Goicoechea De Jorge, R.J.Everett,X.Bai,R.M.Exley,P.N.Ward,N.Ruivo,K.Trivedi,E.Cumber, R.Jones,L.Newham,D.Staunton,R.Borrow,M.Pickering,S.M.Lea,C.M.Tang

Key ref:

S.Johnson et al. (2012). Design and evaluation of meningococcal vaccines through structure-based modification of host and pathogen molecules. Plos Pathog, 8, e1002981. PubMed id: 23133374

Date:

20-Jun-12 Release date: 07-Nov-12

Protein chains

P08603  (CFAH_HUMAN) -  Complement factor H from Homo sapiens

Seq: 1231 a.a.

Struc: 123 a.a.*

Protein chain

P08603  (CFAH_HUMAN) -  Complement factor H from Homo sapiens

Seq: 1231 a.a.

Struc: 117 a.a.*

Protein chains

Q9JXV4  (Q9JXV4_NEIMB) -  Factor H binding protein from Neisseria meningitidis serogroup B (strain ATCC BAA-335 / MC58)

Seq: 274 a.a.

Struc: 238 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Plos Pathog 8:e1002981 (2012)

PubMed id: 23133374

Design and evaluation of meningococcal vaccines through structure-based modification of host and pathogen molecules.

S.Johnson, L.Tan, S.van der Veen, J.Caesar, E.Goicoechea De Jorge, R.J.Harding, X.Bai, R.M.Exley, P.N.Ward, N.Ruivo, K.Trivedi, E.Cumber, R.Jones, L.Newham, D.Staunton, R.Ufret-Vincenty, R.Borrow, M.C.Pickering, S.M.Lea, C.M.Tang.

ABSTRACT

Neisseria meningitis remains a leading cause of sepsis and meningitis, and vaccines are required to prevent infections by this important human pathogen. Factor H binding protein (fHbp) is a key antigen that elicits protective immunity against the meningococcus and recruits the host complement regulator, fH. As the high affinity interaction between fHbp and fH could impair immune responses, we sought to identify non-functional fHbps that could act as effective immunogens. This was achieved by alanine substitution of fHbps from all three variant groups (V1, V2 and V3 fHbp) of the protein; while some residues affected fH binding in each variant group, the distribution of key amino underlying the interaction with fH differed between the V1, V2 and V3 proteins. The atomic structure of V3 fHbp in complex with fH and of the C-terminal barrel of V2 fHbp provide explanations to the differences in the precise nature of their interactions with fH, and the instability of the V2 protein. To develop transgenic models to assess the efficacy of non-functional fHbps, we determined the structural basis of the low level of interaction between fHbp and murine fH; in addition to changes in amino acids in the fHbp binding site, murine fH has a distinct conformation compared with the human protein that would sterically inhibit binding to fHbp. Non-functional V1 fHbps were further characterised by binding and structural studies, and shown in non-transgenic and transgenic mice (expressing chimeric fH that binds fHbp and precisely regulates complement system) to retain their immunogenicity. Our findings provide a catalogue of non-functional fHbps from all variant groups that can be included in new generation meningococcal vaccines, and establish proof-in-principle for clinical studies to compare their efficacy with wild-type fHbps.