PDBsum entry 4h42

Hydrolase/inhibitor

PDB id: 4h42

Contents

Protein chain

248 a.a.

Ligands

11E

PG6

Waters ×132

PDB id:

4h42

Name:

Hydrolase/inhibitor

Title:

Synthesis of a weak basic upa inhibitor and crystal structure of complex with upa

Structure:

Urokinase-type plasminogen activator. Chain: u. Fragment: human urokinase-type plasminogen activator catalytic domain. Synonym: u-plasminogen activator, upa, urokinase-type plasminogen activator long chain a, urokinase-type plasminogen activator short chain a, urokinase-type plasminogen activator chain b. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: plau. Expressed in: pichia pastoris. Expression_system_taxid: 4922.

Resolution:

2.01Å R-factor: 0.211 R-free: 0.285

Authors:

H.-Y.Yu,D.Gao,X.Zhang,L.-G.Jiang,Z.-B.Hong,C.Yuan,X.Fang,J.-D.Wang, M.-D.Huang

Key ref:

H.-Y.Yu et al. (2013). Synthesis of a weak basic upa inhibitor and crystal structure of complex with upa. Chin j struct chem, 32, 961.

Date:

14-Sep-12 Release date: 30-Oct-13

Protein chain

P00749  (UROK_HUMAN) -  Urokinase-type plasminogen activator from Homo sapiens

Seq: 431 a.a.

Struc: 248 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.21.73 - u-plasminogen activator.
• Reaction: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.