PDBsum entry 3pmw

Transport protein

PDB id: 3pmw

Contents

Protein chain

261 a.a. *

Ligands

GLU

SO4 ×7

GOL ×2

DMS

G69

Waters ×148

* Residue conservation analysis

PDB id:

3pmw

Name:

Transport protein

Title:

Ligand-binding domain of glua2 (flip) ionotropic glutamate receptor in complex with an allosteric modulator

Structure:

Glutamate receptor 2. Chain: a. Fragment: ligand binding domain, residues 413 to 527 and 653 to 796. Synonym: glur-2, ampa-selective glutamate receptor 2, glur-b, glur- k2, glutamate receptor ionotropic, ampa 2, glua2. Engineered: yes. Mutation: yes. Other_details: s1-s2 fusion in which gly118 and thr119 replace a membrane-spanning region

Source:

Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: glur2, gria2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.20Å R-factor: 0.229 R-free: 0.313

Authors:

J.K.F.Maclean,C.Jamieson,C.I.Brown,R.A.Campbell,K.J.Gillen, J.Gillespie,B.Kazemier,M.Kiczun,Y.Lamont,A.J.Lyons,E.M.Moir, J.A.Morrow,J.Pantling,Z.Rankovic,L.Smith

Key ref:

C.Jamieson et al. (2011). Structure based evolution of a novel series of positive modulators of the AMPA receptor. Bioorg Med Chem Lett, 21, 805-811. PubMed id: 21190850

Date:

18-Nov-10 Release date: 12-Jan-11

Protein chain

P19491  (GRIA2_RAT) -  Glutamate receptor 2 from Rattus norvegicus

Seq: 883 a.a.

Struc: 261 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Bioorg Med Chem Lett 21:805-811 (2011)

PubMed id: 21190850

Structure based evolution of a novel series of positive modulators of the AMPA receptor.

C.Jamieson, J.K.Maclean, C.I.Brown, R.A.Campbell, K.J.Gillen, J.Gillespie, B.Kazemier, M.Kiczun, Y.Lamont, A.J.Lyons, E.M.Moir, J.A.Morrow, J.Pantling, Z.Rankovic, L.Smith.

ABSTRACT

Starting from compound 1, we utilized biostructural data to successfully evolve an existing series into a new chemotype with a promising overall profile, exemplified by 19.