PDBsum entry 3pb1

Hydrolase inhibitor/hydrolase

PDB id: 3pb1

Contents

Protein chains

375 a.a. *

247 a.a. *

Ligands

SO4 ×2

Waters ×314

* Residue conservation analysis

PDB id:

3pb1

Name:

Hydrolase inhibitor/hydrolase

Title:

Crystal structure of a michaelis complex between plasminogen activator inhibitor-1 and urokinase-type plasminogen activator

Structure:

Plasminogen activator inhibitor 1. Chain: i. Synonym: pai, pai-1, endothelial plasminogen activator inhibitor, serpin e1. Engineered: yes. Mutation: yes. Plasminogen activator, urokinase. Chain: e. Fragment: unp residues 143-395.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: serpine1, pai1, planh1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: plau, rp11-417o11.1-002. Expressed in: pichia pastoris. Expression_system_taxid: 4922

Resolution:

2.30Å R-factor: 0.223 R-free: 0.272

Authors:

Z.Lin,L.Jiang,M.Huang,Structure 2 Function Project (S2f)

Key ref:

Z.Lin et al. (2011). Structural basis for recognition of urokinase-type plasminogen activator by plasminogen activator inhibitor-1. J Biol Chem, 286, 7027-7032. PubMed id: 21199867

Date:

20-Oct-10 Release date: 29-Dec-10

Protein chain

P05121  (PAI1_HUMAN) -  Plasminogen activator inhibitor 1 from Homo sapiens

Seq: 402 a.a.

Struc: 375 a.a.*

Protein chain

P00749  (UROK_HUMAN) -  Urokinase-type plasminogen activator from Homo sapiens

Seq: 431 a.a.

Struc: 247 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.73 - u-plasminogen activator.
• Reaction: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

J Biol Chem 286:7027-7032 (2011)

PubMed id: 21199867

Structural basis for recognition of urokinase-type plasminogen activator by plasminogen activator inhibitor-1.

Z.Lin, L.Jiang, C.Yuan, J.K.Jensen, X.Zhang, Z.Luo, B.C.Furie, B.Furie, P.A.Andreasen, M.Huang.

ABSTRACT

No abstract given.