PDBsum entry 3omh

Hydrolase

PDB id: 3omh

Contents

Protein chains

294 a.a.

Ligands

ASP-GLY-GLU-GLU-PTR-ASP-ASP-PRO-PHE ×3

ASP-GLY-GLU-GLU-PTR-ASP-ASP-PRO

Waters ×140

PDB id:

3omh

Name:

Hydrolase

Title:

Crystal structure of ptpn22 in complex with skap-hom ptyr75 peptide

Structure:

Tyrosine-protein phosphatase non-receptor type 22. Chain: a, b, c, d. Fragment: tyrosine-protein phosphatase domain residues 1-294. Synonym: hematopoietic cell protein-tyrosine phosphatase 70z-pep, lymphoid phosphatase, lyp. Engineered: yes. Mutation: yes. Src kinase-associated phosphoprotein 2. Chain: e, f, g, h.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ptpn22, ptpn8. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: this sequence occurs naturally in humans.

Resolution:

2.90Å R-factor: 0.229 R-free: 0.260

Authors:

X.Yu,J.-P.Sun,S.Zhang,Z.-Y.Zhang

Key ref:

X.Yu et al. (2011). Substrate specificity of lymphoid-specific tyrosine phosphatase (Lyp) and identification of Src kinase-associated protein of 55 kDa homolog (SKAP-HOM) as a Lyp substrate. J Biol Chem, 286, 30526-30534. PubMed id: 21719704

Date:

26-Aug-10 Release date: 29-Jun-11

Protein chains

Q9Y2R2  (PTN22_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 22 from Homo sapiens

Seq: 807 a.a.

Struc: 294 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

J Biol Chem 286:30526-30534 (2011)

PubMed id: 21719704

Substrate specificity of lymphoid-specific tyrosine phosphatase (Lyp) and identification of Src kinase-associated protein of 55 kDa homolog (SKAP-HOM) as a Lyp substrate.

X.Yu, M.Chen, S.Zhang, Z.H.Yu, J.P.Sun, L.Wang, S.Liu, T.Imasaki, Y.Takagi, Z.Y.Zhang.

ABSTRACT

No abstract given.