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PDBsum entry 3ohm
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protein ligands metals Protein-protein interface(s) links
Signaling protein / hydrolase PDB id
3ohm

 

 

 

 

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Contents
Protein chains
318 a.a. *
759 a.a. *
Ligands
GDP-ALF
ACT ×3
Metals
_MG
_CA
Waters ×227
* Residue conservation analysis
PDB id:
3ohm
Name: Signaling protein / hydrolase
Title: Crystal structure of activated g alpha q bound to its effector phospholipasE C beta 3
Structure: Guanine nucleotide-binding protein g(q) subunit alpha. Chain: a. Fragment: unp residues 35-359. Synonym: guanine nucleotide-binding protein alpha-q. Engineered: yes. 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3. Chain: b. Fragment: unp residues 10-886.
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: gnaq. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: high five. Homo sapiens. Human.
Resolution:
2.70Å     R-factor:   0.207     R-free:   0.275
Authors: G.L.Waldo,J.Sondek,T.K.Harden
Key ref: G.L.Waldo et al. (2010). Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex. Science, 330, 974-980. PubMed id: 20966218
Date:
17-Aug-10     Release date:   27-Oct-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P21279  (GNAQ_MOUSE) -  Guanine nucleotide-binding protein G(q) subunit alpha from Mus musculus
Seq:
Struc: 		359 a.a.
318 a.a.
Protein chain
Pfam   ArchSchema ?
Q01970  (PLCB3_HUMAN) -  1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1234 a.a.
759 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain B: E.C.3.1.4.11  - phosphoinositide phospholipase C.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway: 		myo-Inositol Phosphate Metabolism
      Reaction: a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H+
1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)
 + H2O
 = 1D-myo-inositol 1,4,5-trisphosphate
 + 1,2-diacyl-sn-glycerol
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    Added reference    
 
 
Science 330:974-980 (2010)
PubMed id: 20966218  
 
 
Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex.
G.L.Waldo, T.K.Ricks, S.N.Hicks, M.L.Cheever, T.Kawano, K.Tsuboi, X.Wang, C.Montell, T.Kozasa, J.Sondek, T.K.Harden.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21822282 A.M.Lyon, V.M.Tesmer, V.D.Dhamsania, D.M.Thal, J.Gutierrez, S.Chowdhury, K.C.Suddala, J.K.Northup, and J.J.Tesmer (2011).
An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation.
  Nat Struct Mol Biol, 18, 999.
PDB codes: 3qr0 3qr1
21915140 W.H.Moolenaar, and A.Perrakis (2011).
Insights into autotaxin: how to produce and present a lipid mediator.
  Nat Rev Mol Cell Biol, 12, 674-679.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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