PDBsum entry 3ncb

Hormone/hormone receptor

PDB id: 3ncb

Contents

Protein chains

186 a.a. *

206 a.a. *

Ligands

CO3

Metals

_NA ×3

_CL

Waters ×321

* Residue conservation analysis

PDB id:

3ncb

Name:

Hormone/hormone receptor

Title:

A mutant human prolactin receptor antagonist h180a in complex with the extracellular domain of the human prolactin receptor

Structure:

Prolactin. Chain: a. Fragment: sequence database residues 43-227. Synonym: prl. Engineered: yes. Mutation: yes. Prolactin receptor. Chain: b. Fragment: extracellular domain residues 26-234.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hprl, prl. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: hprlr, prlr.

Resolution:

2.10Å R-factor: 0.188 R-free: 0.237

Authors:

M.V.Kulkarni,M.C.Tettamanzi,J.W.Murphy,C.Keeler,D.G.Myszka, N.E.Chayen,E.J.Lolis,M.E.Hodsdon

Key ref:

M.V.Kulkarni et al. (2010). Two independent histidines, one in human prolactin and one in its receptor, are critical for pH-dependent receptor recognition and activation. J Biol Chem, 285, 38524-38533. PubMed id: 20889499

Date:

04-Jun-10 Release date: 29-Sep-10

Protein chain

P01236  (PRL_HUMAN) -  Prolactin from Homo sapiens

Seq: 227 a.a.

Struc: 186 a.a.*

Protein chain

P16471  (PRLR_HUMAN) -  Prolactin receptor from Homo sapiens

Seq: 622 a.a.

Struc: 206 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

J Biol Chem 285:38524-38533 (2010)

PubMed id: 20889499

Two independent histidines, one in human prolactin and one in its receptor, are critical for pH-dependent receptor recognition and activation.

M.V.Kulkarni, M.C.Tettamanzi, J.W.Murphy, C.Keeler, D.G.Myszka, N.E.Chayen, E.J.Lolis, M.E.Hodsdon.

ABSTRACT

No abstract given.