 |
PDBsum entry 3ldz
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Protein transport
|
PDB id
|
|
|
|
3ldz
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Vhs domains of escrt-0 cooperate in high-Avidity binding to polyubiquitinated cargo.
|
|
|
Authors
|
|
X.Ren,
J.H.Hurley.
|
|
|
Ref.
|
|
Embo J, 2010,
29,
1045-1054.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA
adapters, and other trafficking proteins. The structure of the STAM VHS
domain-ubiquitin complex was solved at 2.6 A resolution, revealing that
determinants for ubiquitin recognition are conserved in nearly all VHS domains.
VHS domains from all classes of VHS-domain containing proteins in yeast and
humans, including both subunits of ESCRT-0, bound ubiquitin in vitro. ESCRTs
have been implicated in the sorting of Lys63-linked polyubiquitinated cargo.
Intact human ESCRT-0 binds Lys63-linked tetraubiquitin 50-fold more tightly than
monoubiquitin, though only 2-fold more tightly than Lys48-linked tetraubiquitin.
The gain in affinity is attributed to the cooperation of flexibly connected VHS
and UIM motifs of ESCRT-0 in avid binding to the polyubiquitin chain. Mutational
analysis of all the five ubiquitin-binding sites in yeast ESCRT-0 shows that
cooperation between them is required for the sorting of the Lys63-linked
polyubiquitinated cargo Cps1 to the vacuole.
|
|
|
|
|
|
|
