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PDBsum entry 3jcm
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Transcription
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PDB id
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3jcm
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Contents |
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2174 a.a.
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429 a.a.
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416 a.a.
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734 a.a.
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279 a.a.
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139 a.a.
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126 a.a.
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843 a.a.
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1686 a.a.
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79 a.a.
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73 a.a.
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77 a.a.
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90 a.a.
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72 a.a.
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70 a.a.
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70 a.a.
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71 a.a.
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65 a.a.
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92 a.a.
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77 a.a.
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74 a.a.
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77 a.a.
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66 a.a.
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77 a.a.
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References listed in PDB file
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Key reference
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Title
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The 3.8 å structure of the u4/u6.U5 tri-Snrnp: insights into spliceosome assembly and catalysis.
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Authors
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R.Wan,
C.Yan,
R.Bai,
L.Wang,
M.Huang,
C.C.Wong,
Y.Shi.
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Ref.
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Science, 2016,
351,
466-475.
[DOI no: ]
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PubMed id
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Abstract
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Splicing of precursor messenger RNA is accomplished by a dynamic megacomplex
known as the spliceosome. Assembly of a functional spliceosome requires a
preassembled U4/U6.U5 tri-snRNP complex, which comprises the U5 small nuclear
ribonucleoprotein (snRNP), the U4 and U6 small nuclear RNA (snRNA) duplex, and a
number of protein factors. Here we report the three-dimensional structure of a
Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at an overall resolution of 3.8
angstroms by single-particle electron cryomicroscopy. The local resolution for
the core regions of the tri-snRNP reaches 3.0 to 3.5 angstroms, allowing
construction of a refined atomic model. Our structure contains U5 snRNA, the
extensively base-paired U4/U6 snRNA, and 30 proteins including Prp8 and Snu114,
which amount to 8495 amino acids and 263 nucleotides with a combined molecular
mass of ~1 megadalton. The catalytic nucleotide U80 from U6 snRNA exists in an
inactive conformation, stabilized by its base-pairing interactions with U4 snRNA
and protected by Prp3. Pre-messenger RNA is bound in the tri-snRNP through
base-pairing interactions with U6 snRNA and loop I of U5 snRNA. This structure,
together with that of the spliceosome, reveals the molecular choreography of the
snRNAs in the activation process of the spliceosomal ribozyme.
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