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PDBsum entry 3j5m
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Viral protein/immune system
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PDB id
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3j5m
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Contents |
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415 a.a.
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63 a.a.
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208 a.a.
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225 a.a.
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References listed in PDB file
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Key reference
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Title
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Cryo-Em structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer.
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Authors
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D.Lyumkis,
J.P.Julien,
N.De val,
A.Cupo,
C.S.Potter,
P.J.Klasse,
D.R.Burton,
R.W.Sanders,
J.P.Moore,
B.Carragher,
I.A.Wilson,
A.B.Ward.
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Ref.
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Science, 2013,
342,
1484-1490.
[DOI no: ]
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PubMed id
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Abstract
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The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites
and membrane fusion machinery that introduce the viral genome into the host
cell. As the only target for broadly neutralizing antibodies (bnAbs), Env is a
focus for rational vaccine design. We present a cryo-electron microscopy
reconstruction and structural model of a cleaved, soluble Env trimer (termed
BG505 SOSIP.664 gp140) in complex with a CD4 binding site (CD4bs) bnAb, PGV04,
at 5.8 angstrom resolution. The structure reveals the spatial arrangement of Env
components, including the V1/V2, V3, HR1, and HR2 domains, as well as shielding
glycans. The structure also provides insights into trimer assembly, gp120-gp41
interactions, and the CD4bs epitope cluster for bnAbs, which covers a more
extensive area and defines a more complex site of vulnerability than previously
described.
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