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PDBsum entry 3he7
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Immune system
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PDB id
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3he7
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Contents |
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281 a.a.
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97 a.a.
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197 a.a.
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240 a.a.
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References listed in PDB file
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Key reference
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Title
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Differential recognition of cd1d-Alpha-Galactosyl ceramide by the V beta 8.2 and V beta 7 semi-Invariant nkt t cell receptors.
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Authors
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D.G.Pellicci,
O.Patel,
L.Kjer-Nielsen,
S.S.Pang,
L.C.Sullivan,
K.Kyparissoudis,
A.G.Brooks,
H.H.Reid,
S.Gras,
I.S.Lucet,
R.Koh,
M.J.Smyth,
T.Mallevaey,
J.L.Matsuda,
L.Gapin,
J.Mccluskey,
D.I.Godfrey,
J.Rossjohn.
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Ref.
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Immunity, 2009,
31,
47-59.
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PubMed id
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Abstract
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The semi-invariant natural killer T cell receptor (NKT TCR) recognizes
CD1d-lipid antigens. Although the TCR alpha chain is typically invariant, the
beta chain expression is more diverse, where three V beta chains are commonly
expressed in mice. We report the structures of V alpha 14-V beta 8.2 and V alpha
14-V beta 7 NKT TCRs in complex with CD1d-alpha-galactosylceramide
(alpha-GalCer) and the 2.5 A structure of the human NKT TCR-CD1d-alpha-GalCer
complex. Both V beta 8.2 and V beta 7 NKT TCRs and the human NKT TCR ligated
CD1d-alpha-GalCer in a similar manner, highlighting the evolutionarily conserved
interaction. However, differences within the V beta domains of the V beta 8.2
and V beta 7 NKT TCR-CD1d complexes resulted in altered TCR beta-CD1d-mediated
contacts and modulated recognition mediated by the invariant alpha chain.
Mutagenesis studies revealed the differing contributions of V beta 8.2 and V
beta 7 residues within the CDR2 beta loop in mediating contacts with CD1d.
Collectively we provide a structural basis for the differential NKT TCR V beta
usage in NKT cells.
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