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PDBsum entry 3gtk
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Transcription, transferase/DNA-RNA hybri
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PDB id
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3gtk
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Contents |
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1442 a.a.
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1153 a.a.
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271 a.a.
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215 a.a.
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85 a.a.
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136 a.a.
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119 a.a.
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65 a.a.
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114 a.a.
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46 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of transcription: backtracked RNA polymerase ii at 3.4 angstrom resolution.
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Authors
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D.Wang,
D.A.Bushnell,
X.Huang,
K.D.Westover,
M.Levitt,
R.D.Kornberg.
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Ref.
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Science, 2009,
324,
1203-1206.
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PubMed id
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Abstract
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Transcribing RNA polymerases oscillate between three stable states, two of
which, pre- and posttranslocated, were previously subjected to x-ray crystal
structure determination. We report here the crystal structure of RNA polymerase
II in the third state, the reverse translocated, or "backtracked" state. The
defining feature of the backtracked structure is a binding site for the first
backtracked nucleotide. This binding site is occupied in case of nucleotide
misincorporation in the RNA or damage to the DNA, and is termed the "P" site
because it supports proofreading. The predominant mechanism of proofreading is
the excision of a dinucleotide in the presence of the elongation factor SII
(TFIIS). Structure determination of a cocrystal with TFIIS reveals a
rearrangement whereby cleavage of the RNA may take place.
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