PDBsum entry 3ftj

Hydrolase

PDB id

3ftj

Loading ...

Contents

			Protein chain

					214 a.a. *

			Waters ×139

* Residue conservation analysis

PDB id:

3ftj

Links

Name:

Hydrolase

Title:

Crystal structure of the periplasmic region of macb from actinobacillus actinomycetemcomitans

Structure:

Macrolide export atp-binding/permease protein macb. Chain: a. Fragment: periplasmic region, unp residues 293-518. Synonym: macb. Engineered: yes

Source:

Actinobacillus actinomycetemcomitans. Haemophilus actinomycetemcomitans. Organism_taxid: 714. Gene: macb, macb_actac. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å

R-factor:

0.202

R-free:

0.250

Authors:

Y.Xu,N.C.Ha

Key ref:

Y.Xu et al. (2009). Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter. Biochemistry, 48, 5218-5225. PubMed id: 19432486

Date:

13-Jan-09

Release date:

26-May-09

PROCHECK

	Headers

	References

Protein chain

Q2EHL8 (MACB_AGGAC) - Macrolide export ATP-binding/permease protein MacB from Aggregatibacter actinomycetemcomitans

Seq: Struc:

Seq: Struc:					644 a.a. 214 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class:

E.C.7.6.2.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Biochemistry 48:5218-5225 (2009)
PubMed id: 19432486

Crystal structure of the periplasmic region of MacB, a noncanonic ABC transporter.
Y.Xu, S.H.Sim, K.H.Nam, X.L.Jin, H.M.Kim, K.Y.Hwang, K.Lee, N.C.Ha.

ABSTRACT

MacB is a noncanonic ABC-type transporter within Gram-negative bacteria, which is responsible both for the efflux of macrolide antibiotics and for the secretion of heat-stable enterotoxin II. In Escherichia coli, MacB requires the membrane fusion protein MacA and the multifunctional outer membrane channel TolC to pump substrates to the external medium. Sequence analysis of MacB suggested that MacB has a relatively large periplasmic region. To gain insight into how MacB assembles with MacA and TolC, we determined the crystal structure of the periplasmic region of Actinobacillus actinomycetemcomitans MacB. Fold matching program reveals that parts of the MacB periplasmic region have structural motifs in common with the RND-type transporter AcrB. Since it behaved as a monomer in solution, our finding is consistent with the dimeric nature of full-length MacB, providing an insight into the assembly in the tripartite efflux pump.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20581201

H.M.Kim, Y.Xu, M.Lee, S.Piao, S.H.Sim, N.C.Ha, and K.Lee (2010).
Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC.

J Bacteriol, 192, 4498-4503.

19722844

H.I.Zgurskaya (2009).
Multicomponent drug efflux complexes: architecture and mechanism of assembly.

Future Microbiol, 4, 919-932.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.