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PDBsum entry 3ftj
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the periplasmic region of macb, A noncanonic abc transporter.
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Authors
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Y.Xu,
S.H.Sim,
K.H.Nam,
X.L.Jin,
H.M.Kim,
K.Y.Hwang,
K.Lee,
N.C.Ha.
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Ref.
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Biochemistry, 2009,
48,
5218-5225.
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PubMed id
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Abstract
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MacB is a noncanonic ABC-type transporter within Gram-negative bacteria, which
is responsible both for the efflux of macrolide antibiotics and for the
secretion of heat-stable enterotoxin II. In Escherichia coli, MacB requires the
membrane fusion protein MacA and the multifunctional outer membrane channel TolC
to pump substrates to the external medium. Sequence analysis of MacB suggested
that MacB has a relatively large periplasmic region. To gain insight into how
MacB assembles with MacA and TolC, we determined the crystal structure of the
periplasmic region of Actinobacillus actinomycetemcomitans MacB. Fold matching
program reveals that parts of the MacB periplasmic region have structural motifs
in common with the RND-type transporter AcrB. Since it behaved as a monomer in
solution, our finding is consistent with the dimeric nature of full-length MacB,
providing an insight into the assembly in the tripartite efflux pump.
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