PDBsum entry 3d1n

Transcription regulator/DNA

PDB id: 3d1n

Contents

Protein chains

150 a.a. *

86 a.a. *

DNA/RNA

Waters ×97

* Residue conservation analysis

PDB id:

3d1n

Name:

Transcription regulator/DNA

Title:

Structure of human brn-5 transcription factor in complex with corticotrophin-releasing hormone gene promoter

Structure:

5'-d( Dap Dgp Dcp Dap Dtp Dap Dap Dap Dtp Dap Dap Dtp Dap D a)-3'. Chain: a, c, e, g. Engineered: yes. 5'-d( Dtp Dtp Dap Dtp Dtp Dap Dtp Dtp Dtp Dap Dtp Dgp Dcp D t)-3'. Chain: b, d, f, h. Engineered: yes. Pou domain, class 6, transcription factor 1.

Source:

Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Gene: pou6f1, brn5, mpou, tcfb1. Expressed in: escherichia coli.

Resolution:

2.51Å R-factor: 0.212 R-free: 0.270

Authors:

J.H.Pereira,S.C.Ha,S.-H.Kim

Key ref:

J.H.Pereira and S.H.Kim (2009). Structure of human Brn-5 transcription factor in complex with CRH gene promoter. J Struct Biol, 167, 159-165. PubMed id: 19450691

Date:

06-May-08 Release date: 26-May-09

Protein chains

Q14863  (PO6F1_HUMAN) -  POU domain, class 6, transcription factor 1 from Homo sapiens

Seq: 301 a.a.

Struc: 150 a.a.*

Protein chains

Q14863  (PO6F1_HUMAN) -  POU domain, class 6, transcription factor 1 from Homo sapiens

Seq: 301 a.a.

Struc: 86 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

DNA/RNA chains

A-G-C-A-T-A-A-A-T-A-A-T-A-A 14 bases

T-T-A-T-T-A-T-T-T-A-T-G-C-T 14 bases

A-G-C-A-T-A-A-A-T-A-A-T-A-A 14 bases

T-T-A-T-T-A-T-T-T-A-T-G-C-T 14 bases

A-G-C-A-T-A-A-A-T-A-A-T-A-A 14 bases

T-T-A-T-T-A-T-T-T-A-T-G-C-T 14 bases

A-G-C-A-T-A-A-A-T-A-A-T-A-A 14 bases

T-T-A-T-T-A-T-T-T-A-T-G-C-T 14 bases

J Struct Biol 167:159-165 (2009)

PubMed id: 19450691

Structure of human Brn-5 transcription factor in complex with CRH gene promoter.

J.H.Pereira, S.H.Kim.

ABSTRACT

The Brn-5 protein, highly expressed in human brain, belongs to the POU family; a class of transcription factors involved in a wide variety of biological processes ranging from programming of embryonic stem cells to cellular housekeeping. This functional diversity is conferred by two DNA-binding subdomains that can assume several configurations due to a bipartite arrangement of POU-specific (POU(S)) and POU-homeo (POU(H)) subdomains separated by a linker region. The crystal structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone (CRH) gene promoter reveals an unexpected recognition mode of the protein to its cognate DNA. Moreover, the structure also shows the role of the linker in allowing diverse configurations that can be assumed by the two subdomains.