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PDBsum entry 3d1n

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protein dna_rna Protein-protein interface(s) links
Transcription regulator/DNA PDB id
3d1n
Jmol
Contents
Protein chains
150 a.a. *
86 a.a. *
DNA/RNA
Waters ×97
* Residue conservation analysis
PDB id:
3d1n
Name: Transcription regulator/DNA
Title: Structure of human brn-5 transcription factor in complex with corticotrophin-releasing hormone gene promoter
Structure: 5'- d( Dap Dgp Dcp Dap Dtp Dap Dap Dap Dtp Dap Dap Dtp Dap Da)- 3'. Chain: a, c, e, g. Engineered: yes. 5'- d( Dtp Dtp Dap Dtp Dtp Dap Dtp Dtp Dtp Dap Dtp Dgp Dcp Dt)- 3'. Chain: b, d, f, h.
Source: Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Gene: pou6f1, brn5, mpou, tcfb1. Expressed in: escherichia coli.
Resolution:
2.51Å     R-factor:   0.212     R-free:   0.270
Authors: J.H.Pereira,S.C.Ha,S.-H.Kim
Key ref: J.H.Pereira and S.H.Kim (2009). Structure of human Brn-5 transcription factor in complex with CRH gene promoter. J Struct Biol, 167, 159-165. PubMed id: 19450691 DOI: 10.1016/j.jsb.2009.05.003
Date:
06-May-08     Release date:   26-May-09    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q14863  (PO6F1_HUMAN) -  POU domain, class 6, transcription factor 1
Seq:
Struc:
301 a.a.
150 a.a.*
Protein chains
Pfam   ArchSchema ?
Q14863  (PO6F1_HUMAN) -  POU domain, class 6, transcription factor 1
Seq:
Struc:
301 a.a.
86 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 8 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     regulation of transcription, DNA-dependent   1 term 
  Biochemical function     DNA binding     2 terms  

 

 
DOI no: 10.1016/j.jsb.2009.05.003 J Struct Biol 167:159-165 (2009)
PubMed id: 19450691  
 
 
Structure of human Brn-5 transcription factor in complex with CRH gene promoter.
J.H.Pereira, S.H.Kim.
 
  ABSTRACT  
 
The Brn-5 protein, highly expressed in human brain, belongs to the POU family; a class of transcription factors involved in a wide variety of biological processes ranging from programming of embryonic stem cells to cellular housekeeping. This functional diversity is conferred by two DNA-binding subdomains that can assume several configurations due to a bipartite arrangement of POU-specific (POU(S)) and POU-homeo (POU(H)) subdomains separated by a linker region. The crystal structure of human Brn-5 transcription factor in complex with corticotrophin-releasing hormone (CRH) gene promoter reveals an unexpected recognition mode of the protein to its cognate DNA. Moreover, the structure also shows the role of the linker in allowing diverse configurations that can be assumed by the two subdomains.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20334529 R.Rohs, X.Jin, S.M.West, R.Joshi, B.Honig, and R.S.Mann (2010).
Origins of specificity in protein-DNA recognition.
  Annu Rev Biochem, 79, 233-269.  
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