PDBsum entry 3cfp

Transferase/DNA

PDB id: 3cfp

Contents

Protein chain

901 a.a. *

DNA/RNA

Ligands

TTP

Metals

_CA ×4

_CL

Waters ×299

* Residue conservation analysis

PDB id:

3cfp

Name:

Transferase/DNA

Title:

Structure of the replicating complex of a pol alpha family DNA polymerase, ternary complex 1

Structure:

DNA (5'- d( Dap Dcp Dap Dgp Dgp Dtp Dap Dap Dgp Dcp Dap Dgp Dtp Dcp Dcp Dgp Dc p Dg)-3'). Chain: t. Engineered: yes. Other_details: substrate DNA, template strand. DNA (5'- d( Dgp Dcp Dgp Dgp Dap Dcp Dtp Dgp Dcp Dtp Dtp Dap Dcp (Doc))-3'). Chain: p.

Source:

Synthetic: yes. Other_details: synthesized by w.M. Keck facility at yale university). Bacteriophage rb69. Gene: 43. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.200 R-free: 0.267

Authors:

J.Wang,D.Klimenko,M.Wang,T.A.Steitz,W.H.Konigsberg

Key ref:

D.Klimenko et al. To be published, .

Date:

04-Mar-08 Release date: 10-Mar-09

Protein chain

Q38087  (DPOL_BPR69) -  DNA-directed DNA polymerase from Escherichia phage RB69

Seq: 903 a.a.

Struc: 901 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

A-C-A-G-G-T-A-A-G-C-A-G-T-C-C-G-C-G 18 bases

G-C-G-G-A-C-T-G-C-T-T-A-C-DOC 14 bases

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: E.C.3.1.11.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site