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PDBsum entry 3c5z
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Sugar binding protein/immune system
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PDB id
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3c5z
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Contents |
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202 a.a.
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240 a.a.
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182 a.a.
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202 a.a.
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References listed in PDB file
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Key reference
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Title
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Crossreactive t cells spotlight the germline rules for alphabeta t cell-Receptor interactions with mhc molecules.
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Authors
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S.Dai,
E.S.Huseby,
K.Rubtsova,
J.Scott-Browne,
F.Crawford,
W.A.Macdonald,
P.Marrack,
J.W.Kappler.
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Ref.
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Immunity, 2008,
28,
324-334.
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PubMed id
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Abstract
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To test whether highly crossreactive alphabeta T cell receptors (TCRs) produced
during limited negative selection best illustrate evolutionarily conserved
interactions between TCR and major histocompatibility complex (MHC) molecules,
we solved the structures of three TCRs bound to the same MHC II peptide
(IAb-3K). The TCRs had similar affinities for IAb-3K but varied from
noncrossreactive to extremely crossreactive with other peptides and MHCs.
Crossreactivity correlated with a shrinking, increasingly hydrophobic TCR-ligand
interface, involving fewer TCR amino acids. A few CDR1 and CDR2 amino acids
dominated the most crossreactive TCR interface with MHC, including Vbeta8 48Y
and 54E and Valpha4 29Y, arranged to impose the familiar diagonal orientation of
TCR on MHC. These interactions contribute to MHC binding by other TCRs using
related V regions, but not usually so dominantly. These data show that
crossreactive TCRs can spotlight the evolutionarily conserved features of
TCR-MHC interactions and that these interactions impose the diagonal docking of
TCRs on MHC.
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