PDBsum entry 3wiv

Hydrolase

PDB id: 3wiv

Contents

Protein chains

391 a.a.

Metals

_CA ×18

Waters ×833

PDB id:

3wiv

Name:

Hydrolase

Title:

Crystal structure of pro-s324a/d356a

Structure:

Tk-subtilisin. Chain: a, b, c. Fragment: unp residues 25-422. Engineered: yes. Mutation: yes

Source:

Thermococcus kodakarensis. Organism_taxid: 69014. Strain: atcc baa-918 / jcm 12380 / kod1. Gene: tk1675. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.177 R-free: 0.220

Authors:

R.Uehara,C.Angkawidjaja,Y.Koga,S.Kanaya

Key ref:

R.Uehara et al. Requirement of the insertion sequence for activation tk-Subtilisin. To be published, .

Date:

25-Sep-13 Release date: 01-Oct-14

Protein chains

P58502  (TKSU_THEKO) -  Tk-subtilisin from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

Seq: 422 a.a.

Struc: 391 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.21.- - ?????