PDBsum entry 3m61

Hydrolase/hydrolase inhibitor

PDB id: 3m61

Contents

Protein chains

247 a.a. *

12 a.a. *

Waters ×129

* Residue conservation analysis

PDB id:

3m61

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of complex of urokinase and a upain-1 variant(w3a) in ph4.6 condition

Structure:

Urokinase-type plasminogen activator. Chain: u. Fragment: c-terminal domain, unp residues 179-431. Synonym: u-plasminogen activator, upa, urokinase-type plasminogen activator chain b. Engineered: yes. Mutation: yes. Upain-1 w3a. Chain: p.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: plau. Expressed in: pichia pastoris. Expression_system_taxid: 4922. Synthetic: yes. Other_details: synthetic construct

Resolution:

1.68Å R-factor: 0.198 R-free: 0.243

Authors:

L.Jiang,C.Yuan,T.Wind,P.A.Andreasen,L.Chen,E.J.Meehan,M.Huang

Key ref:

L.Jiang et al. Crystal structure of complex of urokinase and a upain-1 variant(w3a) in ph4.6 condition. To be published, .

Date:

15-Mar-10 Release date: 07-Apr-10

Protein chain

P00749  (UROK_HUMAN) -  Urokinase-type plasminogen activator from Homo sapiens

Seq: 431 a.a.

Struc: 247 a.a.*

Protein chain

No UniProt id for this chain

Struc: 12 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain U: E.C.3.4.21.73 - u-plasminogen activator.
• Reaction: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.