PDBsum entry 2x19

Nuclear transport

PDB id

2x19

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Contents

			Protein chains

					172 a.a. *


					907 a.a. *

			Ligands

					GTP

			Metals

					_MG

			Waters ×58

* Residue conservation analysis

PDB id:

2x19

Links

Name:

Nuclear transport

Title:

Crystal structure of importin13 - rangtp complex

Structure:

Gtp-binding nuclear protein gsp1/cnr1. Chain: a. Fragment: residues 8-179. Synonym: gtpase ran homolog, genetic suppressor of prp20-1, chromosome stability protein 17, ran. Engineered: yes. Mutation: yes. Importin-13. Chain: b.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 511693. Homo sapiens. Human. Organism_taxid: 9606.

Resolution:

2.80Å

R-factor:

0.236

R-free:

0.268

Authors:

F.Bono,A.G.Cook,M.Gruenwald,J.Ebert,E.Conti

Key ref:

F.Bono et al. (2010). Nuclear import mechanism of the EJC component Mago-Y14 revealed by structural studies of importin 13. Mol Cell, 37, 211-222. PubMed id: 20122403

Date:

23-Dec-09

Release date:

16-Feb-10

PROCHECK

	Headers

	References

Protein chain

P32835 (GSP1_YEAST) - GTP-binding nuclear protein GSP1/CNR1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					219 a.a. 172 a.a.*

Protein chain

O94829 (IPO13_HUMAN) - Importin-13 from Homo sapiens

Seq: Struc:

Seq: Struc:					963 a.a. 907 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

	Mol Cell 37:211-222 (2010)
PubMed id: 20122403

Nuclear import mechanism of the EJC component Mago-Y14 revealed by structural studies of importin 13.
F.Bono, A.G.Cook, M.Grünwald, J.Ebert, E.Conti.

ABSTRACT

Mago and Y14 are core components of the exon junction complex (EJC), an assembly central to nonsense-mediated mRNA decay in humans and mRNA localization in flies. The Mago-Y14 heterodimer shuttles between the nucleus, where it is loaded onto specific mRNAs, and the cytoplasm, where it functions in translational regulation. The heterodimer is imported back into the nucleus by Importin 13 (Imp13), a member of the karyopherin-beta family of transport factors. We have elucidated the structural basis of the Mago-Y14 nuclear import cycle. The 3.35 A structure of the Drosophila Imp13-Mago-Y14 complex shows that Imp13 forms a ring-like molecule, reminiscent of Crm1, and encircles the Mago-Y14 cargo with a conserved interaction surface. The 2.8 A structure of human Imp13 bound to RanGTP reveals how Mago-Y14 is released in the nucleus by a steric hindrance mechanism. Comparison of the two structures suggests how this unusual karyopherin might function in bidirectional nucleocytoplasmic transport.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21139563

M.Grünwald, and F.Bono (2011).
Structure of Importin13-Ubc9 complex: nuclear import and release of a key regulator of sumoylation.

EMBO J, 30, 427-438.

PDB code:

2xwu

20479275

G.Buchwald, J.Ebert, C.Basquin, J.Sauliere, U.Jayachandran, F.Bono, H.Le Hir, and E.Conti (2010).
Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.

Proc Natl Acad Sci U S A, 107, 10050-10055.

PDB code:

2xb2

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.