PDBsum entry 2w8c

Electron transport

PDB id: 2w8c

Contents

Protein chains

106 a.a. *

Metals

_ZN ×4

_CU ×2

Waters ×160

* Residue conservation analysis

PDB id:

2w8c

Name:

Electron transport

Title:

Plastocyanin variant with n-terminal methionine - closed structure

Structure:

Plastocyanin. Chain: a, b. Engineered: yes

Source:

Phormidium laminosum. Organism_taxid: 32059. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.80Å R-factor: 0.176 R-free: 0.224

Authors:

P.B.Crowley,P.M.Matias,A.R.Khan

Key ref:

P.B.Crowley et al. (2009). Metal-mediated self-assembly of a beta-sandwich protein. Chemistry, 15, 12672-12680. PubMed id: 19834935

Date:

15-Jan-09 Release date: 27-Oct-09

Protein chains

Q51883  (PLAS_LEPLM) -  Plastocyanin from Leptolyngbya laminosa

Seq: 139 a.a.

Struc: 106 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Chemistry 15:12672-12680 (2009)

PubMed id: 19834935

Metal-mediated self-assembly of a beta-sandwich protein.

P.B.Crowley, P.M.Matias, A.R.Khan, M.Roessle, D.I.Svergun.

ABSTRACT

The beta-sandwich cupredoxin Plastocyanin (Pc) was found to self-assemble in the presence of Zn(2+), a known mediator of protein-protein interfaces. Diffraction-quality crystals of Pc grew from solutions containing zinc acetate as the sole precipitant. Di- and trinuclear zinc sites contribute to the crystal contacts in this structure. A different crystal form, also involving numerous zinc bridging ions, was obtained in the presence of poly(ethylene glycol) 8 000. Comparison of the two crystal forms reveals the effect of macromolecular crowding on self-assembly. Solution-state structural characterisation of the Zn(2+)-mediated Pc oligomers was performed by using a combination of chemical shift perturbation mapping and small-angle X-ray scattering. The data indicate the formation of dimers in solution. The implications for metal-mediated assembly and crystallisation are discussed.