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PDBsum entry 2w8c
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Electron transport
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PDB id
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2w8c
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References listed in PDB file
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Key reference
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Title
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Metal-Mediated self-Assembly of a beta-Sandwich protein.
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Authors
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P.B.Crowley,
P.M.Matias,
A.R.Khan,
M.Roessle,
D.I.Svergun.
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Ref.
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Chemistry, 2009,
15,
12672-12680.
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PubMed id
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Abstract
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The beta-sandwich cupredoxin Plastocyanin (Pc) was found to self-assemble in the
presence of Zn(2+), a known mediator of protein-protein interfaces.
Diffraction-quality crystals of Pc grew from solutions containing zinc acetate
as the sole precipitant. Di- and trinuclear zinc sites contribute to the crystal
contacts in this structure. A different crystal form, also involving numerous
zinc bridging ions, was obtained in the presence of poly(ethylene glycol) 8 000.
Comparison of the two crystal forms reveals the effect of macromolecular
crowding on self-assembly. Solution-state structural characterisation of the
Zn(2+)-mediated Pc oligomers was performed by using a combination of chemical
shift perturbation mapping and small-angle X-ray scattering. The data indicate
the formation of dimers in solution. The implications for metal-mediated
assembly and crystallisation are discussed.
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