PDBsum entry 2miz

Viral protein

PDB id: 2miz

Contents

Protein chain

156 a.a.

PDB id:

2miz

Name:

Viral protein

Title:

Structure of the m04/gp34 mouse cytomegalovirus immunoevasin core domain

Structure:

M04 immunoevasin. Chain: a. Engineered: yes. Mutation: yes

Source:

Murine cytomegalovirus. Muhv-1. Organism_taxid: 69156. Strain: k181. Gene: m04. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

10 models

Authors:

N.G.Sgourakis,K.Natarajan,D.H.Margulies,A.Bax

Key ref:

N.G.Sgourakis et al. (2014). The structure of mouse cytomegalovirus m04 protein obtained from sparse NMR data reveals a conserved fold of the m02-m06 viral immune modulator family. Structure, 22, 1263-1273. PubMed id: 25126960 DOI: 10.1016/j.str.2014.05.018

Date:

21-Dec-13 Release date: 16-Jul-14

Protein chain

A2Q6L0  (A2Q6L0_MUHVK) -  M04 immunoevasin (Fragment) from Murid herpesvirus 1 (strain K181)

Seq: 266 a.a.

Struc: 156 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/j.str.2014.05.018

Structure 22:1263-1273 (2014)

PubMed id: 25126960

The structure of mouse cytomegalovirus m04 protein obtained from sparse NMR data reveals a conserved fold of the m02-m06 viral immune modulator family.

N.G.Sgourakis, K.Natarajan, J.Ying, B.Vogeli, L.F.Boyd, D.H.Margulies, A.Bax.

ABSTRACT

Immunoevasins are key proteins used by viruses to subvert host immune responses. Determining their high-resolution structures is key to understanding virus-host interactions toward the design of vaccines and other antiviral therapies. Mouse cytomegalovirus encodes a unique set of immunoevasins, the m02-m06 family, that modulates major histocompatibility complex class I (MHC-I) antigen presentation to CD8+ T cells and natural killer cells. Notwithstanding the large number of genetic and functional studies, the structural biology of immunoevasins remains incompletely understood, largely because of crystallization bottlenecks. Here we implement a technology using sparse nuclear magnetic resonance data and integrative Rosetta modeling to determine the structure of the m04/gp34 immunoevasin extracellular domain. The structure reveals a β fold that is representative of the m02-m06 family of viral proteins, several of which are known to bind MHC-I molecules and interfere with antigen presentation, suggesting its role as a diversified immune regulation module.