PDBsum entry 2k0c

Metal binding protein

PDB id

2k0c

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Contents

			Protein chain

					35 a.a. *

			Metals

					_ZN

* Residue conservation analysis

PDB id:

2k0c

Links
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Name:

Metal binding protein

Title:

Zinc-finger 2 of nup153

Structure:

Nuclear pore complex protein nup153. Chain: a. Fragment: ranbp2-type 2 domain (unp residues 703-755). Synonym: nucleoporin nup153, 153 kda nucleoporin. Engineered: yes

Source:

Rattus norvegicus. Rat. Gene: nup153. Expressed in: escherichia coli.

NMR struc:

20 models

Authors:

J.A.Bangert,N.Schrader,I.R.Vetter,R.Stoll

Key ref:

N.Schrader et al. (2008). The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure, 16, 1116-1125. PubMed id: 18611384

Date:

31-Jan-08

Release date:

01-Jul-08

PROCHECK

	Headers

	References

Protein chain

P49791 (NU153_RAT) - Nuclear pore complex protein Nup153 from Rattus norvegicus

Seq: Struc:

Seq: Struc:

Seq: Struc:					1468 a.a. 35 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Structure 16:1116-1125 (2008)
PubMed id: 18611384

The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex.
N.Schrader, C.Koerner, K.Koessmeier, J.A.Bangert, A.Wittinghofer, R.Stoll, I.R.Vetter.

ABSTRACT

Nucleoporin (Nup) 153 is a highly mobile, multifunctional, and essential nuclear pore protein. It contains four zinc finger motifs that are thought to be crucial for the regulation of transport-receptor/cargo interactions via their binding to the small guanine nucleotide binding protein, Ran. We found this interaction to be independent of the phoshorylation state of the nucleotide. Ran binds with the highest affinity to the second zinc finger motif of Nup153 (Nup153ZnF2). Here we present the crystal structure of this complex, revealing a new type of Ran-Ran interaction partner interface together with the solution structure of Nup153ZnF2. According to our complex structure, Nup153ZnF2 binding to Ran excludes the formation of a Ran-importin-beta complex. This finding suggests a local Nup153-mediated Ran reservoir at the nucleoplasmic distal ring of the nuclear pore, where nucleotide exchange may take place in a ternary Nup153-Ran-RCC1 complex, so that import complexes are efficiently terminated.

Literature references that cite this PDB file's key reference

PubMed id

Reference

19525551

J.A.DeGrasse, K.N.DuBois, D.Devos, T.N.Siegel, A.Sali, M.C.Field, M.P.Rout, and B.T.Chait (2009).
Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor.

Mol Cell Proteomics, 8, 2119-2130.

19505478

J.R.Partridge, and T.U.Schwartz (2009).
Crystallographic and biochemical analysis of the Ran-binding zinc finger domain.

J Mol Biol, 391, 375-389.

PDB codes:

3gj0 3gj3 3gj4 3gj5 3gj6 3gj7 3gj8

19748337

S.G.Brohawn, J.R.Partridge, J.R.Whittle, and T.U.Schwartz (2009).
The nuclear pore complex has entered the atomic age.

Structure, 17, 1156-1168.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.