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PDBsum entry 2k0c
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Metal binding protein
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PDB id
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2k0c
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References listed in PDB file
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Key reference
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Title
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The crystal structure of the ran-Nup153znf2 complex: a general ran docking site at the nuclear pore complex.
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Authors
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N.Schrader,
C.Koerner,
K.Koessmeier,
J.A.Bangert,
A.Wittinghofer,
R.Stoll,
I.R.Vetter.
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Ref.
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Structure, 2008,
16,
1116-1125.
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PubMed id
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Abstract
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Nucleoporin (Nup) 153 is a highly mobile, multifunctional, and essential nuclear
pore protein. It contains four zinc finger motifs that are thought to be crucial
for the regulation of transport-receptor/cargo interactions via their binding to
the small guanine nucleotide binding protein, Ran. We found this interaction to
be independent of the phoshorylation state of the nucleotide. Ran binds with the
highest affinity to the second zinc finger motif of Nup153 (Nup153ZnF2). Here we
present the crystal structure of this complex, revealing a new type of Ran-Ran
interaction partner interface together with the solution structure of
Nup153ZnF2. According to our complex structure, Nup153ZnF2 binding to Ran
excludes the formation of a Ran-importin-beta complex. This finding suggests a
local Nup153-mediated Ran reservoir at the nucleoplasmic distal ring of the
nuclear pore, where nucleotide exchange may take place in a ternary
Nup153-Ran-RCC1 complex, so that import complexes are efficiently terminated.
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