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PDBsum entry 2han
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Transcription/DNA
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PDB id
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2han
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References listed in PDB file
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Key reference
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Title
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Novel DNA-Binding element within the c-Terminal extension of the nuclear receptor DNA-Binding domain.
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Authors
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M.Jakób,
R.Kołodziejczyk,
M.Orłowski,
S.Krzywda,
A.Kowalska,
J.Dutko-Gwóźdź,
T.Gwóźdź,
M.Kochman,
M.Jaskólski,
A.Ozyhar.
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Ref.
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Nucleic Acids Res, 2007,
35,
2705-2718.
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PubMed id
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Abstract
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The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members
of the nuclear receptors superfamily, is considered as the functional receptor
for ecdysteroids initiating molting and metamorphosis in insects. Here we report
the 1.95 A structure of the complex formed by the DNA-binding domains (DBDs) the
EcR and the Usp, bound to the natural pseudopalindromic response element.
Comparison of the structure with that obtained previously, using an idealized
response element, shows how the EcRDBD, which has been previously reported to
possess extraordinary flexibility, accommodates DNA-induced structural changes.
Part of the C-terminal extension (CTE) of the EcRDBD folds into an alpha-helix
whose location in the minor groove does not match any of the locations
previously observed for nuclear receptors. Mutational analyses suggest that the
alpha-helix is a component of EcR-box, a novel element indispensable for
DNA-binding and located within the nuclear receptor CTE. This element seems to
be a general feature of all known EcRs.
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