PDBsum entry 2qyi

Hydrolase/hydrolase inhibitor

PDB id: 2qyi

Contents

Protein chains

223 a.a. *

175 a.a. *

Metals

_CA ×2

_NI

Waters ×312

* Residue conservation analysis

PDB id:

2qyi

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of a binary complex between an engineered trypsin inhibitor and bovine trypsin

Structure:

Cationic trypsin. Chain: a, c. Synonym: beta-trypsin. Engineered: yes. Chymotrypsin inhibitor 3. Chain: b, d. Synonym: wci-3. Engineered: yes. Mutation: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Expressed in: escherichia coli. Expression_system_taxid: 562. Psophocarpus tetragonolobus. Goa bean. Organism_taxid: 3891. Expression_system_taxid: 562

Resolution:

2.60Å R-factor: 0.208 R-free: 0.268

Authors:

S.Khamrui,J.Dasgupta,J.K.Dattagupta,U.Sen

Key ref:

U.Sen Crystal structure of a binary complex between an engineered trypsin inhibitor and bovine trypsin. To be published, .

Date:

15-Aug-07 Release date: 19-Aug-08

Protein chains

P00760  (TRY1_BOVIN) -  Serine protease 1 from Bos taurus

Seq: 246 a.a.

Struc: 223 a.a.

Protein chains

P10822  (ICW3_PSOTE) -  Chymotrypsin inhibitor 3 from Psophocarpus tetragonolobus

Seq: 207 a.a.

Struc: 175 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, C: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.