PDBsum entry 1vto

Transcription/DNA

PDB id: 1vto

Contents

Protein chains

187 a.a.

DNA/RNA

Waters ×1561

References listed in PDB file

Key reference

• Title: 1.9 a resolution refined structure of tbp recognizing the minor groove of tataaaag.
• Authors: J.L.Kim, S.K.Burley.
• Ref.: Nat Struct Biol, 1994, 1, 638-653.
• PubMed id: 7634103

Abstract

The three-dimensional structure of a TATA box-binding protein (TBP) from Arabidopsis thaliana complexed with a fourteen base pair oligonucleotide bearing the Adenovirus major late promoter TATA element has been refined at 1.9 A resolution, giving a final crystallographic R-factor of 19.4%. Binding of the monomeric, saddle-shaped alpha/beta protein induces an unprecedented conformational change in the DNA. A detailed structural and functional analysis of this unusual protein-DNA complex is presented, with particular emphasis on the mechanisms of DNA deformation, TATA element recognition, and preinitiation complex assembly.