PDBsum entry 1qlh

Oxidoreductase

PDB id: 1qlh

Contents

Protein chain

374 a.a. *

Ligands

NAD

Metals

_ZN ×2

Waters ×88

* Residue conservation analysis

PDB id:

1qlh

Name:

Oxidoreductase

Title:

Horse liver alcohol dehydrogenase complexed to NAD double mutant of gly 293 ala and pro 295 thr

Structure:

Alcohol dehydrogenase. Chain: a. Synonym: adh, alcohol dehydrogenase e chain. Engineered: yes. Mutation: yes

Source:

Equus caballus. Horse. Organism_taxid: 9796. Organ: liver. Cellular_location: cytoplasm. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: e.Coli expressed mutant protein

Biol. unit:

Dimer (from PDB file)

Resolution:

2.07Å R-factor: 0.209 R-free: 0.254

Authors:

S.Ramaswamy,B.V.Plapp

Date:

31-Aug-99 Release date: 02-Jan-00

Protein chain

P00327  (ADH1E_HORSE) -  Alcohol dehydrogenase E chain from Equus caballus

Seq: 375 a.a.

Struc: 374 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.1.1.1 - alcohol dehydrogenase.
• Reaction:
  1. a primary alcohol + NAD⁺ = an aldehyde + NADH + H⁺
  2. a secondary alcohol + NAD⁺ = a ketone + NADH + H⁺

primary alcohol + NAD(+) (Bound ligand (Het Group name = NAD) matches with 97.73% similarity) = aldehyde + NADH + H(+)

secondary alcohol + NAD(+) (Bound ligand (Het Group name = NAD) matches with 97.73% similarity) = ketone + NADH + H(+)

• Cofactor: Zn(2+) or Fe cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site