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PDBsum entry 1qat
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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C2 domain conformational changes in phospholipase c-Delta 1.
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Authors
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J.A.Grobler,
L.O.Essen,
R.L.Williams,
J.H.Hurley.
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Ref.
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Nat Struct Biol, 1996,
3,
788-795.
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PubMed id
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Abstract
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The structure of the PH-domain truncated core of rat phosphoinositide-specific
phospholipase C-delta 1 has been determined at 2.4 A resolution and compared to
the structure previously determined in a different crystal form. The
stereochemical relationship between the EF, catalytic, and C2 domains is
essentially identical. The Ca2+ analogue Sm3+ binds at two sites between the
jaws of the C2 domain. Sm3+ binding ejects three lysine residues which bridge
the gap between the jaws and occupy the Ca2+ site in the apoenzyme, triggering a
conformational change in the jaws. The distal sections of the C2 jaws move
apart, opening the mouth by 9 A and creating a gap large enough to bind a
phospholipid headgroup.
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Secondary reference #1
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Title
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Crystal structure of a mammalian phosphoinositide-Specific phospholipase c delta.
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Authors
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L.O.Essen,
O.Perisic,
R.Cheung,
M.Katan,
R.L.Williams.
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Ref.
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Nature, 1996,
380,
595-602.
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PubMed id
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Secondary reference #2
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Title
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Expression, Characterization, And crystallization of the catalytic core of rat phosphatidylinositide-Specific phospholipase c delta 1.
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Authors
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J.A.Grobler,
J.H.Hurley.
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Ref.
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Protein Sci, 1996,
5,
680-686.
[DOI no: ]
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PubMed id
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