PDBsum entry 1ntv

Signaling protein

PDB id

1ntv

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Contents

			Protein chain

					152 a.a. *

			Ligands

					ASN-PHE-ASP-ASN- PRO-VAL-TYR-ARG- LYS-THR


					PO4

			Waters ×139

* Residue conservation analysis

PDB id:

1ntv

Links

Name:

Signaling protein

Title:

Crystal structure of the disabled-1 (dab1) ptb domain-apoer2 peptide complex

Structure:

Disabled homolog 1. Chain: a. Fragment: residues 23-174. Engineered: yes. Apolipoprotein e receptor-2 peptide. Chain: b. Engineered: yes

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Gene: dab1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: chemically synthesized

Biol. unit:

Dimer (from PQS)

Resolution:

1.50Å

R-factor:

0.222

R-free:

0.245

Authors:

P.C.Stolt,H.Jeon,H.K.Song,J.Herz,M.J.Eck,S.C.Blacklow

Key ref:

P.C.Stolt et al. (2003). Origins of peptide selectivity and phosphoinositide binding revealed by structures of disabled-1 PTB domain complexes. Structure, 11, 569-579. PubMed id: 12737822

Date:

30-Jan-03

Release date:

15-Apr-03

PROCHECK

	Headers

	References

Protein chain

P97318 (DAB1_MOUSE) - Disabled homolog 1 from Mus musculus

Seq: Struc:

Seq: Struc:					588 a.a. 152 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

	Structure 11:569-579 (2003)
PubMed id: 12737822

Origins of peptide selectivity and phosphoinositide binding revealed by structures of disabled-1 PTB domain complexes.
P.C.Stolt, H.Jeon, H.K.Song, J.Herz, M.J.Eck, S.C.Blacklow.

ABSTRACT

Formation of the mammalian six-layered neocortex depends on a signaling pathway that involves Reelin, the very low-density lipoprotein receptor, the apolipoprotein E receptor-2 (ApoER2), and the adaptor protein Disabled-1 (Dab1). The 1.5 A crystal structure of a complex between the Dab1 phosphotyrosine binding (PTB) domain and a 14-residue peptide from the ApoER2 tail explains the unusual preference of Dab1 for unphosphorylated tyrosine within the NPxY motif of the peptide. Crystals of the complex soaked with the phosphoinositide PI-4,5P(2) (PI) show that PI binds to conserved basic residues on the PTB domain opposite the peptide binding groove. This finding explains how the Dab1 PTB domain can simultaneously bind PI and the ApoER2 tail. Recruitment of the Dab1 PTB domain to PI-rich regions of the plasma membrane may facilitate association with the Reelin receptor cytoplasmic tails to transduce a critical positional cue to migrating neurons.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20868222

M.Dieckmann, M.F.Dietrich, and J.Herz (2010).
Lipoprotein receptors--an evolutionarily ancient multifunctional receptor family.

Biol Chem, 391, 1341-1363.

20568118

O.Y.Kwon, K.Hwang, J.A.Kim, K.Kim, I.C.Kwon, H.K.Song, and H.Jeon (2010).
Dab1 binds to Fe65 and diminishes the effect of Fe65 or LRP1 on APP processing.

J Cell Biochem, 111, 508-519.

20053677

X.Tong, D.Zitserman, I.Serebriiskii, M.Andrake, R.Dunbrack, and F.Roegiers (2010).
Numb independently antagonizes Sanpodo membrane targeting and Notch signaling in Drosophila sensory organ precursor cells.

Mol Biol Cell, 21, 802-810.

19482617

K.N.Pandey (2009).
Functional roles of short sequence motifs in the endocytosis of membrane receptors.

Front Biosci, 14, 5339-5360.

18550529

J.Radzimanowski, S.Ravaud, S.Schlesinger, J.Koch, K.Beyreuther, I.Sinning, and K.Wild (2008).
Crystal structure of the human Fe65-PTB1 domain.

J Biol Chem, 283, 23113-23120.

PDB codes:

3d8d 3d8e 3d8f

17922498

M.Leone, E.C.Yu, R.C.Liddington, E.B.Pasquale, and M.Pellecchia (2008).
The PTB domain of tensin: NMR solution structure and phosphoinositides binding studies.

Biopolymers, 89, 86-92.

PDB code:

2gjy

18843039

P.A.Keyel, J.R.Thieman, R.Roth, E.Erkan, E.T.Everett, S.C.Watkins, J.E.Heuser, and L.M.Traub (2008).
The AP-2 adaptor beta2 appendage scaffolds alternate cargo endocytosis.

Mol Biol Cell, 19, 5309-5326.

17588168

A.Rosenhouse-Dantsker, and D.E.Logothetis (2007).
Molecular characteristics of phosphoinositide binding.

Pflugers Arch, 455, 45-53.

17473008

C.J.McCleverty, D.C.Lin, and R.C.Liddington (2007).
Structure of the PTB domain of tensin1 and a model for its recruitment to fibrillar adhesions.

Protein Sci, 16, 1223-1229.

PDB code:

1wvh

17339315

D.F.Ceccarelli, I.M.Blasutig, M.Goudreault, Z.Li, J.Ruston, T.Pawson, and F.Sicheri (2007).
Non-canonical interaction of phosphoinositides with pleckstrin homology domains of Tiam1 and ArhGAP9.

J Biol Chem, 282, 13864-13874.

PDB codes:

2p0d 2p0f 2p0h

17373875

E.Im, and A.Kazlauskas (2007).
PtdIns-4,5-P2 as a potential therapeutic target for pathologic angiogenesis.

Expert Opin Ther Targets, 11, 443-451.

17502098

J.Li, X.Mao, L.Q.Dong, F.Liu, and L.Tong (2007).
Crystal structures of the BAR-PH and PTB domains of human APPL1.

Structure, 15, 525-533.

PDB codes:

2ela 2elb

17363896

R.K.Mahadev, S.M.Di Pietro, J.M.Olson, H.L.Piao, G.S.Payne, and M.Overduin (2007).
Structure of Sla1p homology domain 1 and interaction with the NPFxD endocytic internalization motif.

EMBO J, 26, 1963-1971.

PDB code:

2hbp

16519655

E.Förster, Y.Jossin, S.Zhao, X.Chai, M.Frotscher, and A.M.Goffinet (2006).
Recent progress in understanding the role of Reelin in radial neuronal migration, with specific emphasis on the dentate gyrus.

Eur J Neurosci, 23, 901-909.

16951405

H.S.Hoe, T.S.Tran, Y.Matsuoka, B.W.Howell, and G.W.Rebeck (2006).
DAB1 and Reelin effects on amyloid precursor protein and ApoE receptor 2 trafficking and processing.

J Biol Chem, 281, 35176-35185.

17053810

J.Herz, and Y.Chen (2006).
Reelin, lipoprotein receptors and synaptic plasticity.

Nat Rev Neurosci, 7, 850-859.

16728974

X.Shi, T.Hong, K.L.Walter, M.Ewalt, E.Michishita, T.Hung, D.Carney, P.Peña, F.Lan, M.R.Kaadige, N.Lacoste, C.Cayrou, F.Davrazou, A.Saha, B.R.Cairns, D.E.Ayer, T.G.Kutateladze, Y.Shi, J.Côté, K.F.Chua, and O.Gozani (2006).
ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression.

Nature, 442, 96-99.

15952897

H.Jeon, and S.C.Blacklow (2005).
Structure and physiologic function of the low-density lipoprotein receptor.

Annu Rev Biochem, 74, 535-562.

16046028

M.Xu, L.Arnaud, and J.A.Cooper (2005).
Both the phosphoinositide and receptor binding activities of Dab1 are required for Reelin-stimulated Dab1 tyrosine phosphorylation.

Brain Res Mol Brain Res, 139, 300-305.

15632144

P.C.Stolt, Y.Chen, P.Liu, H.H.Bock, S.C.Blacklow, and J.Herz (2005).
Phosphoinositide binding by the disabled-1 PTB domain is necessary for membrane localization and Reelin signal transduction.

J Biol Chem, 280, 9671-9677.

16179341

R.Garuti, C.Jones, W.P.Li, P.Michaely, J.Herz, R.D.Gerard, J.C.Cohen, and H.H.Hobbs (2005).
The modular adaptor protein autosomal recessive hypercholesterolemia (ARH) promotes low density lipoprotein receptor clustering into clathrin-coated pits.

J Biol Chem, 280, 40996-41004.

15728179

S.K.Mishra, P.A.Keyel, M.A.Edeling, A.L.Dupin, D.J.Owen, and L.M.Traub (2005).
Functional dissection of an AP-2 beta2 appendage-binding sequence within the autosomal recessive hypercholesterolemia protein.

J Biol Chem, 280, 19270-19280.

16102539

U.Beffert, E.J.Weeber, A.Durudas, S.Qiu, I.Masiulis, J.D.Sweatt, W.P.Li, G.Adelmann, M.Frotscher, R.E.Hammer, and J.Herz (2005).
Modulation of synaptic plasticity and memory by Reelin involves differential splicing of the lipoprotein receptor Apoer2.

Neuron, 47, 567-579.

15473838

D.J.Owen, B.M.Collins, and P.R.Evans (2004).
Adaptors for clathrin coats: structure and function.

Annu Rev Cell Dev Biol, 20, 153-191.

15175346

H.H.Bock, Y.Jossin, P.May, O.Bergner, and J.Herz (2004).
Apolipoprotein E receptors are required for reelin-induced proteasomal degradation of the neuronal adaptor protein Disabled-1.

J Biol Chem, 279, 33471-33479.

15023338

J.W.Yu, J.M.Mendrola, A.Audhya, S.Singh, D.Keleti, D.B.DeWald, D.Murray, S.D.Emr, and M.A.Lemmon (2004).
Genome-wide analysis of membrane targeting by S. cerevisiae pleckstrin homology domains.

Mol Cell, 13, 677-688.

15066634

M.S.Robinson (2004).
Adaptable adaptors for coated vesicles.

Trends Cell Biol, 14, 167-174.

15452113

N.R.Blatner, R.V.Stahelin, K.Diraviyam, P.T.Hawkins, W.Hong, D.Murray, and W.Cho (2004).
The molecular basis of the differential subcellular localization of FYVE domains.

J Biol Chem, 279, 53818-53827.

14578346

T.G.Kutateladze, D.G.Capelluto, C.G.Ferguson, M.L.Cheever, A.G.Kutateladze, G.D.Prestwich, and M.Overduin (2004).
Multivalent mechanism of membrane insertion by the FYVE domain.

J Biol Chem, 279, 3050-3057.

12906822

A.Farooq, L.Zeng, K.S.Yan, K.S.Ravichandran, and M.M.Zhou (2003).
Coupling of folding and binding in the PTB domain of the signaling protein Shc.

Structure, 11, 905-913.

PDB codes:

1n3h 1oy2

12882964

H.H.Bock, Y.Jossin, P.Liu, E.Förster, P.May, A.M.Goffinet, and J.Herz (2003).
Phosphatidylinositol 3-kinase interacts with the adaptor protein Dab1 in response to Reelin signaling and is required for normal cortical lamination.

J Biol Chem, 278, 38772-38779.

12737812

K.S.Yan, and M.M.Zhou (2003).
Examining both sides of a Janus PTB domain.

Structure, 11, 482-484.

14581447

L.M.Traub (2003).
Sorting it out: AP-2 and alternate clathrin adaptors in endocytic cargo selection.

J Cell Biol, 163, 203-208.

12826668

M.Yun, L.Keshvara, C.G.Park, Y.M.Zhang, J.B.Dickerson, J.Zheng, C.O.Rock, T.Curran, and H.W.Park (2003).
Crystal structures of the Dab homology domains of mouse disabled 1 and 2.

J Biol Chem, 278, 36572-36581.

PDB codes:

1m7e 1oqn 1p3r

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.