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PDBsum entry 1jkf
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The crystal structure and mechanism of 1-L-Myo-Inositol- 1-Phosphate synthase.
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Authors
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A.J.Stein,
J.H.Geiger.
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Ref.
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J Biol Chem, 2002,
277,
9484-9491.
[DOI no: ]
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PubMed id
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Abstract
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1-l-myo-Inositol-1-phosphate synthase catalyzes the conversion of d-glucose
6-phosphate to 1-l-myo-inositol-1-phosphate (MIP), the first and rate-limiting
step in the biosynthesis of all inositol-containing compounds. It involves an
oxidation, intramolecular aldol cyclization, and reduction. We have determined
the first crystal structure of MIP synthase. We present structures of both the
NAD-bound enzyme and the enzyme bound to an inhibitor,
2-deoxy-glucitol-6-phosphate. While 58 amino acids are disordered in the unbound
form of the enzyme in the vicinity of the active site, the inhibitor nucleates
the folding of this domain in a striking example of induced fit, serving to
completely encapsulate it within the enzyme. Three helices and a long
beta-strand are formed in this process. We postulate a mechanism for the
conversion based on the structure of the inhibitor-bound complex.
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Figure 2.
Fig. 2. Ribbon model of the MIP synthase tetramer in the
absence of inhibitor. This and all other structure figures were
made using the program RIBBONS (55). The top two monomers are
colored red and blue, make up one asymmetric unit, and are
related by a noncrystallographic 2-fold axis that is roughly in
the plane of the page. The bottom two monomers are green and are
related to the top two monomers by a crystallographic 2-fold
axis running roughly perpendicular to the page.
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Figure 6.
Fig. 6. Proposed mechanism for the transformation
catalyzed by MIP synthase.
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The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(2002,
277,
9484-9491)
copyright 2002.
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Secondary reference #1
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Title
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Structural studies of mip synthase.
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Authors
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A.J.Stein,
J.H.Geiger.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2000,
56,
348-350.
[DOI no: ]
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PubMed id
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Figure 2.
Figure 2 Crystal images. (a) MIP synthase crystal form I. Note:
crystal nucleated around a fiber. (b) MIP synthase crystal form
II. (c) MIP synthase crystals produced via microseeding at a
dilution of 10^-6.5.
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The above figure is
reproduced from the cited reference
with permission from the IUCr
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