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PDBsum entry 1h0h
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Electron transport
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PDB id
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1h0h
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Gene sequence and the 1.8 a crystal structure of the tungsten-Containing formate dehydrogenase from desulfovibrio gigas.
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Authors
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H.Raaijmakers,
S.Macieira,
J.M.Dias,
S.Teixeira,
S.Bursakov,
R.Huber,
J.J.Moura,
I.Moura,
M.J.Romão.
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Ref.
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Structure, 2002,
10,
1261-1272.
[DOI no: ]
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PubMed id
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Abstract
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Desulfovibrio gigas formate dehydrogenase is the first representative of a
tungsten-containing enzyme from a mesophile that has been structurally
characterized. It is a heterodimer of 110 and 24 kDa subunits. The large
subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate reductase,
center. No small subunit ortholog containing
clusters has been reported. The structural homology with E. coli
FDH-H shows that the essential residues (SeCys158, His159, and Arg407) at the
active site are conserved. The active site is accessible via a positively
charged tunnel, while product release may be facilitated, for H(+) by buried
waters and protonable amino acids and for CO(2) through a hydrophobic channel.
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Figure 3.
Figure 3. Stereo Representation of the Overall Structure of
DgW-FDH from D. gigasThe small domain (214 amino acid residues)
is represented in purple, and the large domain (977 amino acid
residues) is colored after its domain classification. Domain I
(red) corresponds to residues 9-68, 575-603, and 647-734; domain
II (green) contains residues 69-157, 410-574, and 604-646;
domain III (yellow) is defined by residues 157-409; domain IV
(blue) is defined by residues 735-977. Domain IV can be divided
into two subdomains, IVa (residues 735-795) and IVb (residues
796-977). The insertions (relative to the homologous NAP and
FDH-H structures) present in several domains are shown in
gray.(B) Representation of the four "exploded" domains of the
large 977 aa subunit.(C) Comparison of the domain organization
of DgW-FDH, D. desulfuricans NAP, and E. coli FDH-H. The
insertions in DgW-FDH are depicted as dashed areas.(D) Stereo Ca
trace numbered every 20 residues for domains II and III
(top).(E) Stereo Ca trace numbered every 20 residues for domains
I and IV and for the small subunit (below).
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2002,
10,
1261-1272)
copyright 2002.
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Secondary reference #1
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Title
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Tungsten-Containing formate dehydrogenase from desulfovibrio gigas: metal identification and preliminary structural data by multi-Wavelength crystallography.
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Authors
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H.Raaijmakers,
S.Teixeira,
J.M.Dias,
M.J.Almendra,
C.D.Brondino,
I.Moura,
J.J.Moura,
M.J.Romão.
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Ref.
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J Biol Inorg Chem, 2001,
6,
398-404.
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PubMed id
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