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PDBsum entry 1fai
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Immunoglobulin
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PDB id
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1fai
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Contents |
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* Residue conservation analysis
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DOI no:
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Proc Natl Acad Sci U S A
89:9429-9433
(1992)
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PubMed id:
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Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody.
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M.B.Lascombe,
P.M.Alzari,
R.J.Poljak,
A.Nisonoff.
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ABSTRACT
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The three-dimensional structure of FabR19.9 from a well-characterized
anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray
diffraction techniques in two crystalline forms (I and II) to a resolution of
2.8 and 2.7 A, respectively. Essentially the same tertiary and quaternary
structure of the Fab is observed in the two forms. The major difference resides
in the intermolecular contacts, which are interpreted to favor an irreversible
transition from the metastable form I to the more stable form II. The third
complementarity-determining region of the heavy chain (H3) folds back over the
combining site and requires rearrangement for hapten binding. This dynamic
requirement on H3 is consistent with its mobility in the structure and can
explain hapten binding to an otherwise inaccessible antibody combining site.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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R.Day,
K.P.Lennox,
D.B.Dahl,
M.Vannucci,
and
J.W.Tsai
(2010).
Characterizing the regularity of tetrahedral packing motifs in protein tertiary structure.
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Bioinformatics,
26,
3059-3066.
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T.Ota,
J.P.Rast,
G.W.Litman,
and
C.T.Amemiya
(2003).
Lineage-restricted retention of a primitive immunoglobulin heavy chain isotype within the Dipnoi reveals an evolutionary paradox.
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Proc Natl Acad Sci U S A,
100,
2501-2506.
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M.Brown,
M.A.Schumacher,
G.D.Wiens,
R.G.Brennan,
and
M.B.Rittenberg
(2000).
The structural basis of repertoire shift in an immune response to phosphocholine.
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J Exp Med,
191,
2101-2112.
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PDB code:
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J.L.Pellequer,
and
S.W.Chen
(1997).
Does conformational free energy distinguish loop conformations in proteins?
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Biophys J,
73,
2359-2375.
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P.Bork,
A.K.Downing,
B.Kieffer,
and
I.D.Campbell
(1996).
Structure and distribution of modules in extracellular proteins.
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Q Rev Biophys,
29,
119-167.
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R.R.Joshi
(1996).
A self-organizing cognitive network of antibody repertoire development.
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J Comput Biol,
3,
529-545.
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M.Viswanathan,
J.M.Anchin,
P.R.Droupadi,
C.Mandal,
D.S.Linthicum,
and
S.Subramaniam
(1995).
Structural predictions of the binding site architecture for monoclonal antibody NC6.8 using computer-aided molecular modeling, ligand binding, and spectroscopy.
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Biophys J,
69,
741-753.
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P.J.Johansson,
C.Malone,
W.Swietnicki,
B.M.Dunn,
and
R.C.Williams
(1994).
Fv structure of monoclonal antibody II-481 against herpes simplex virus Fc gamma-binding glycoprotein gE contains immunodominant complementarity determining region epitopes that react with human immunoglobulin M rheumatoid factors.
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J Exp Med,
180,
1873-1888.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
