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PDBsum entry 1fai
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Immunoglobulin
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PDB id
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1fai
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Three-Dimensional structure of two crystal forms of fabr19.9 from a monoclonal anti-Arsonate antibody.
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Authors
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M.B.Lascombe,
P.M.Alzari,
R.J.Poljak,
A.Nisonoff.
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Ref.
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Proc Natl Acad Sci U S A, 1992,
89,
9429-9433.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
89%.
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Abstract
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The three-dimensional structure of FabR19.9 from a well-characterized
anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray
diffraction techniques in two crystalline forms (I and II) to a resolution of
2.8 and 2.7 A, respectively. Essentially the same tertiary and quaternary
structure of the Fab is observed in the two forms. The major difference resides
in the intermolecular contacts, which are interpreted to favor an irreversible
transition from the metastable form I to the more stable form II. The third
complementarity-determining region of the heavy chain (H3) folds back over the
combining site and requires rearrangement for hapten binding. This dynamic
requirement on H3 is consistent with its mobility in the structure and can
explain hapten binding to an otherwise inaccessible antibody combining site.
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Secondary reference #1
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Title
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Three-Dimensional structure of FAB r19.9, A monoclonal murine antibody specific for the p-Azobenzenearsonate group.
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Authors
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M.B.Lascombe,
P.M.Alzari,
G.Boulot,
P.Saludjian,
P.Tougard,
C.Berek,
S.Haba,
E.M.Rosen,
A.Nisonoff,
R.J.Poljak.
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Ref.
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Proc Natl Acad Sci U S A, 1989,
86,
607-611.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Crystallization of the FAB fragments of monoclonal anti-P-Azophenylarsonate antibodies and their complexes with haptens.
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Authors
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R.A.Mariuzza,
A.G.Amit,
G.Boulot,
P.Saludjian,
F.A.Saul,
P.Tougard,
R.J.Poljak,
J.Conger,
E.Lamoyi,
A.Nisonoff.
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Ref.
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J Biol Chem, 1984,
259,
5954-5958.
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PubMed id
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