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PDBsum entry 1aoj
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protein Protein-protein interface(s) links
Signal transduction PDB id
1aoj

 

 

 

 

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Contents
Protein chains
60 a.a. *
Waters ×35
* Residue conservation analysis
PDB id:
1aoj
Name: Signal transduction
Title: The sh3 domain of eps8 exists as a novel intertwined dimer
Structure: Eps8. Chain: a, b. Fragment: sh3 domain. Synonym: src homology domain. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562
Biol. unit: Dimer (from PQS)
Resolution:
2.50Å     R-factor:   0.193     R-free:   0.270
Authors: K.V.R.Kishan,M.E.Newcomer
Key ref: K.V.Kishan et al. (1997). The SH3 domain of Eps8 exists as a novel intertwined dimer. Nat Struct Biol, 4, 739-743. PubMed id: 9303002
Date:
07-Jul-97     Release date:   08-Jul-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q08509  (EPS8_MOUSE) -  Epidermal growth factor receptor kinase substrate 8 from Mus musculus
Seq:
Struc: 		 
Seq:
Struc: 		821 a.a.
60 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Nat Struct Biol 4:739-743 (1997)
PubMed id: 9303002  
 
 
The SH3 domain of Eps8 exists as a novel intertwined dimer.
K.V.Kishan, G.Scita, W.T.Wong, P.P.Di Fiore, M.E.Newcomer.
 
  ABSTRACT  
 
SH3 domains are structurally well-characterized as monomeric modular units of protein structure that mediate protein-protein recognition in numerous signal transduction proteins. The X-ray crystallographic structure of the Eps8 SH3 domain reveals a novel variation of the canonical SH3 fold: the SH3 domain from Eps8 is a dimer formed by strand interchange. In addition, co-immunoprecipitation experiments show that intact Eps8 is multimeric in vivo. Hence, the SH3 domain of Eps8 may represent a dimerization motif.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20351091 H.Wang, M.T.Teh, Y.Ji, V.Patel, S.Firouzabadian, A.A.Patel, J.S.Gutkind, and W.A.Yeudall (2010).
EPS8 upregulates FOXM1 expression, enhancing cell growth and motility.
  Carcinogenesis, 31, 1132-1141.  
20532239 M.Hertzog, F.Milanesi, L.Hazelwood, A.Disanza, H.Liu, E.Perlade, M.G.Malabarba, S.Pasqualato, A.Maiolica, S.Confalonieri, C.Le Clainche, N.Offenhauser, J.Block, K.Rottner, P.P.Di Fiore, M.F.Carlier, N.Volkmann, D.Hanein, and G.Scita (2010).
Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping.
  PLoS Biol, 8, e1000387.  
19168626 P.J.Mintz, M.Cardó-Vila, M.G.Ozawa, A.Hajitou, R.Rangel, L.Guzman-Rojas, D.R.Christianson, M.A.Arap, R.J.Giordano, G.R.Souza, J.Easley, A.Salameh, S.Oliviero, R.R.Brentani, E.Koivunen, W.Arap, and R.Pasqualini (2009).
An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment.
  Proc Natl Acad Sci U S A, 106, 2182-2187.  
17452785 T.C.Terwilliger, R.W.Grosse-Kunstleve, P.V.Afonine, P.D.Adams, N.W.Moriarty, P.Zwart, R.J.Read, D.Turk, and L.W.Hung (2007).
Interpretation of ensembles created by multiple iterative rebuilding of macromolecular models.
  Acta Crystallogr D Biol Crystallogr, 63, 597-610.  
16407060 F.Ding, K.C.Prutzman, S.L.Campbell, and N.V.Dokholyan (2006).
Topological determinants of protein domain swapping.
  Structure, 14, 5.  
16621823 J.Zhao, and J.S.Parkinson (2006).
Mutational analysis of the chemoreceptor-coupling domain of the Escherichia coli chemotaxis signaling kinase CheA.
  J Bacteriol, 188, 3299-3307.  
16369945 M.D.Baker, P.M.Wolanin, and J.B.Stock (2006).
Signal transduction in bacterial chemotaxis.
  Bioessays, 28, 9.  
16644733 M.R.Schiller, K.Chakrabarti, G.F.King, N.I.Schiller, B.A.Eipper, and M.W.Maciejewski (2006).
Regulation of RhoGEF activity by intramolecular and intermolecular SH3 domain interactions.
  J Biol Chem, 281, 18774-18786.
PDB code: 1u3o
16055542 J.Chahine, and M.S.Cheung (2005).
Computational studies of the reversible domain swapping of p13suc1.
  Biophys J, 89, 2693-2700.  
16225669 J.Roffers-Agarwal, J.B.Xanthos, and J.R.Miller (2005).
Regulation of actin cytoskeleton architecture by Eps8 and Abi1.
  BMC Cell Biol, 6, 36.  
15653321 K.Lindorff-Larsen, P.Røgen, E.Paci, M.Vendruscolo, and C.M.Dobson (2005).
Protein folding and the organization of the protein topology universe.
  Trends Biochem Sci, 30, 13-19.  
15880548 L.J.Ball, R.Kühne, J.Schneider-Mergener, and H.Oschkinat (2005).
Recognition of Proline-Rich Motifs by Protein-Protein-Interaction Domains.
  Angew Chem Int Ed Engl, 44, 2852-2869.  
15766521 M.A.Lemmon (2005).
Pleckstrin homology domains: two halves make a hole?
  Cell, 120, 574-576.  
16152647 S.D.Khare, K.C.Wilcox, P.Gong, and N.V.Dokholyan (2005).
Sequence and structural determinants of Cu, Zn superoxide dismutase aggregation.
  Proteins, 61, 617-632.  
14747998 B.Japelj, J.P.Waltho, and R.Jerala (2004).
Comparison of backbone dynamics of monomeric and domain-swapped stefin A.
  Proteins, 54, 500-512.  
15231733 C.L.Kielkopf, S.Lücke, and M.R.Green (2004).
U2AF homology motifs: protein recognition in the RRM world.
  Genes Dev, 18, 1513-1526.  
15123602 S.Yuzawa, K.Ogura, M.Horiuchi, N.N.Suzuki, Y.Fujioka, M.Kataoka, H.Sumimoto, and F.Inagaki (2004).
Solution structure of the tandem Src homology 3 domains of p47phox in an autoinhibited form.
  J Biol Chem, 279, 29752-29760.  
12623012 F.Rousseau, J.W.Schymkowitz, and L.S.Itzhaki (2003).
The unfolding story of three-dimensional domain swapping.
  Structure, 11, 243-251.  
12773374 M.Harkiolaki, M.Lewitzky, R.J.Gilbert, E.Y.Jones, R.P.Bourette, G.Mouchiroud, H.Sondermann, I.Moarefi, and S.M.Feller (2003).
Structural basis for SH3 domain-mediated high-affinity binding between Mona/Gads and SLP-76.
  EMBO J, 22, 2571-2582.
PDB code: 1oeb
11711548 H.Delbrück, G.Ziegelin, E.Lanka, and U.Heinemann (2002).
An Src homology 3-like domain is responsible for dimerization of the repressor protein KorB encoded by the promiscuous IncP plasmid RP4.
  J Biol Chem, 277, 4191-4198.
PDB codes: 1igq 1igu
11839489 M.E.Newcomer (2002).
Protein folding and three-dimensional domain swapping: a strained relationship?
  Curr Opin Struct Biol, 12, 48-53.  
12127568 P.P.Di Fiore, and G.Scita (2002).
Eps8 in the midst of GTPases.
  Int J Biochem Cell Biol, 34, 1178-1183.  
12021428 Y.Liu, and D.Eisenberg (2002).
3D domain swapping: as domains continue to swap.
  Protein Sci, 11, 1285-1299.  
11316885 K.V.Kishan, M.E.Newcomer, T.H.Rhodes, and S.D.Guilliot (2001).
Effect of pH and salt bridges on structural assembly: molecular structures of the monomer and intertwined dimer of the Eps8 SH3 domain.
  Protein Sci, 10, 1046-1055.
PDB codes: 1i07 1i0c
11063574 N.Schiering, E.Casale, P.Caccia, P.Giordano, and C.Battistini (2000).
Dimer formation through domain swapping in the crystal structure of the Grb2-SH2-Ac-pYVNV complex.
  Biochemistry, 39, 13376-13382.
PDB code: 1fyr
  11152127 S.M.Larson, and A.R.Davidson (2000).
The identification of conserved interactions within the SH3 domain by alignment of sequences and structures.
  Protein Sci, 9, 2170-2180.  
9989504 A.M.Bilwes, L.A.Alex, B.R.Crane, and M.I.Simon (1999).
Structure of CheA, a signal-transducing histidine kinase.
  Cell, 96, 131-141.
PDB code: 1b3q
10508163 A.M.Mongioví, P.R.Romano, S.Panni, M.Mendoza, W.T.Wong, A.Musacchio, G.Cesareni, and P.P.Di Fiore (1999).
A novel peptide-SH3 interaction.
  EMBO J, 18, 5300-5309.  
10395945 M.C.Maa, J.R.Lai, R.W.Lin, and T.H.Leu (1999).
Enhancement of tyrosyl phosphorylation and protein expression of eps8 by v-Src.
  Biochim Biophys Acta, 1450, 341-351.  
10545323 T.Shirai, C.Mitsuyama, Y.Niwa, Y.Matsui, H.Hotta, T.Yamane, H.Kamiya, C.Ishii, T.Ogawa, and K.Muramoto (1999).
High-resolution structure of the conger eel galectin, congerin I, in lactose-liganded and ligand-free forms: emergence of a new structure class by accelerated evolution.
  Structure, 7, 1223-1233.
PDB codes: 1c1f 1c1l
9666333 R.B.Russell, and C.P.Ponting (1998).
Protein fold irregularities that hinder sequence analysis.
  Curr Opin Struct Biol, 8, 364-371.  
9566119 D.C.Dalgarno, M.C.Botfield, and R.J.Rickles (1997).
SH3 domains and drug design: ligands, structure, and biological function.
  Biopolymers, 43, 383-400.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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