EC 2.1.1.375 - Nns virus cap methyltransferase.

_EC 2.1.1.375 Nns virus cap methyltransferase.

62 PDB entries

EC 2.-.-.- Transferases. [47,784 PDB entries]

EC 2.1.-.- Transferring one-carbon groups. [5,181 PDB entries]

EC 2.1.1.- Methyltransferases. [4,592 PDB entries]

EC 2.1.1.375 Nns virus cap methyltransferase. [62 PDB entries]

4uci

Reaction: a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl- adenosine in mRNA + 2 S-adenosyl-L-homocysteine + H(+).

5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA
+
2 × S-adenosyl-L-methionine
= 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl- adenosine in mRNA
+
2 × S-adenosyl-L-homocysteine
+ H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site.

Comments: The enzyme from non-segmented negative strain (NNS) viruses (e.g. rhabdoviruses) catalyzes two successive methylations. In higher eukaryotes the two methylations occur in the reverse order and are catalyzed by two different enzymes (cf. Ec 2.1.1.56 and Ec 2.1.1.57) that do not required a specific motif.

Links: [IntEnz] [ExPASy] [KEGG]

There are 62 PDB entries in enzyme class E.C.2.1.1.375

PDB code Protein

4uci

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chains: A, B (361 residues)

Bound ligands: Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine

Het Group GTP is 47.76% similar to enzyme reactant G(5')pppR-RNA

4ucj

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chains: A, B (365 residues)

4uck

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chains: A, B (370 residues)

Bound ligand: Het Group SAM corresponds to enzyme reactant S-adenosyl-L-methionine

4ucl

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chains: A, B (364 residues)

4ucy

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chains: A, B (392 residues)

4ucz

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chains: A, B (381 residues)

Bound ligand: Het Group GTP is 45.00% similar to enzyme product S-adenosyl-L-homocysteine

4ud0

X-ray structure and activities of an essential mononegavirales l- protein domain

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chain: B (350 residues)

Bound ligand: Het Group SAH corresponds to enzyme product S-adenosyl-L-homocysteine

5a22

Structure of the l protein of vesicular stomatitis virus from electron cryomicroscopy

Source: Vesicular stomatitis virus. Organism_taxid: 11276. Strain: indiana. Variant: san juan isolate. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21.

Chain: A (2002 residues)

5chs

N-terminal domain of the vesicular stomatitis virus l protein

Source: Vesicular stomatitis indiana virus. Vsiv. Organism_taxid: 11277. Expressed in: escherichia coli. Expression_system_taxid: 562.

Chains: A, B (294 residues)

6pzk

Cryo-em structure of the respiratory syncytial virus polymerase (l) protein bound by the tetrameric phosphoprotein (p)

Source: Human respiratory syncytial virus a2. Organism_taxid: 11259. Strain: a2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Chain: A (1361 residues)

6u1x

Structure of the vesicular stomatitis virus l protein in complex with its phosphoprotein cofactor (3.0 a resolution)

Source: Vesicular stomatitis indiana virus (strain san juan). Vsiv. Organism_taxid: 11285. Strain: san juan. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expressed in: escherichia coli. Expression_system_taxid: 562

Chain: A (2059 residues)

6u5o

Structure of the human metapneumovirus polymerase bound to the phosphoprotein tetramer

Source: Human metapneumovirus (strain can97-83). Hmpv. Organism_taxid: 694067. Strain: can97-83. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Expression_system_atcc_number: crl-1711.

Chain: L (1354 residues)

6ueb

Structure of rabies sad-b19 l-p complex from cryo-em

Source: Rabies virus (strain sad b19). Rabv. Organism_taxid: 11300. Strain: sad b19. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf-9.

Chain: A (2099 residues)

6uen

Cryo-em structure of the respiratory syncytial virus RNA polymerase

Source: Human respiratory syncytial virus. Organism_taxid: 11250. Gene: l, mz07_64039gpl, mz07_64040gpl. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21. Expression_system_cell_line: sf21

Chain: A (1392 residues)

6v85

Parainfluenza virus 5 l-p complex

Source: Parainfluenza virus 5 (strain w3). Piv5. Organism_taxid: 11208. Strain: w3. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v.

Chain: A (1901 residues)

6v86

Parainfluenza virus 5 l-p complex with an alternate conformation of the cd-mtase-ctd module

Source: Simian virus 5 (strain w3). Piv5. Organism_taxid: 11208. Strain: w3. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v.

Chain: A (1890 residues)

6yu8

RNA methyltransferase of sudan ebola virus

Source: Sudan virus - boniface, sudan,1976. Organism_taxid: 128948. Expressed in: escherichia coli. Expression_system_taxid: 562. Lama glama. Llama. Organism_taxid: 9844

Chain: A (268 residues)

7yer

The structure of ebov l-vp35 complex

Source: Ebola virus. Organism_taxid: 1570291. Expressed in: spodoptera aff. Frugiperda 1 bold-2017. Expression_system_taxid: 2449148. Gene: vp35. Expression_system_taxid: 2449148

Chain: A (1316 residues)

7yes

The structure of ebov l-vp35-RNA complex (state2)

Source: Ebola virus. Organism_taxid: 1570291. Expressed in: spodoptera aff. Frugiperda 1 bold-2017. Expression_system_taxid: 2449148. Expression_system_taxid: 2449148

Chain: A (1369 residues)

7yet

The structure of ebov l-vp35 in complex with suramin

Source: Ebola virus. Organism_taxid: 1570291. Expressed in: spodoptera aff. Frugiperda 1 bold-2017. Expression_system_taxid: 2449148. Gene: vp35. Expression_system_taxid: 2449148

Chain: A (999 residues)

7yot

Cryo-em structure of RNA polymerase in complex with p protein tetramer of newcastle disease virus

Source: Avian orthoavulavirus 1. Organism_taxid: 2560319. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chain: A (1309 residues)

7you

Cryo-em structure of RNA polymerase in complex with p protein tetramer of newcastle disease virus

Source: Avian orthoavulavirus 1. Organism_taxid: 2560319. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chain: A (2059 residues)

7yov

Cryo-em structure of RNA polymerase in complex with p protein tetramer of newcastle disease virus

Source: Avian orthoavulavirus 1. Organism_taxid: 2560319. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chain: A (1310 residues)

8fpi

Co-structure of the respiratory syncytial virus RNA-dependent RNA polymerase with mrk-1

Source: Human respiratory syncytial virus a2. Organism_taxid: 11259. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Expression_system_cell_line: sf9

Chain: A (1357 residues)

8fpj

Co-structure of the human metapneunomovirus RNA-dependent RNA polymerase with mrk-1

Source: Human metapneumovirus. Organism_taxid: 162145. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Expression_system_cell_line: sf9

Chain: A (1345 residues)

8fu3

Structure of respiratory syncytial virus polymerase with novel non- nucleoside inhibitor

Source: Human respiratory syncytial virus a2. Organism_taxid: 11259. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chain: A (1367 residues)

8izl

Structure of the mumps virus l protein bound by phosphoprotein tetramer

Source: Mumps orthorubulavirus. Organism_taxid: 2560602. Strain: jeryl-lynn. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Expression_system_cell_line: sf9

Chain: A (2067 residues)

8izm

Structure of the mumps virus l protein (state2) bound by phosphoprotein tetramer

Source: Mumps virus strain jeryl lynn. Organism_taxid: 11168. Strain: jeryl-lynn. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Expression_system_cell_line: sf9

Chain: A (1350 residues)

8jsl

The structure of ebov l-vp35-RNA complex

Source: Ebola virus. Organism_taxid: 1570291. Gene: l. Expressed in: spodoptera. Expression_system_taxid: 7106. Gene: vp35. Synthetic: yes. Organism_taxid: 1570291

Chain: A (1362 residues)

8jsm

The structure of ebov l-vp35-RNA complex (conformation 1)

Source: Ebola virus. Organism_taxid: 1570291. Gene: l. Expressed in: spodoptera. Expression_system_taxid: 7106. Gene: vp35. Synthetic: yes. Organism_taxid: 1570291

Chain: A (1362 residues)

8jsn

The structure of ebov l-vp35-RNA complex (conformation 2)

Source: Ebola virus. Organism_taxid: 1570291. Gene: l. Expressed in: spodoptera. Expression_system_taxid: 7106. Gene: vp35. Synthetic: yes. Organism_taxid: 1570291

Chain: A (1362 residues)

8kdb

Cryo-em structure of the human parainfluenza virus hpiv3 l-p polymerase in dimeric form

Source: Human respirovirus 3. Organism_taxid: 11216. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21. Expression_system_cell_line: sf21

Chains: A, G (2117 residues)

8kdc

Cryo-em structure of the human parainfluenza virus hpiv3 l-p polymerase in monomeric form

Source: Human respirovirus 3. Organism_taxid: 11216. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf21. Expression_system_cell_line: sf21

Chain: A (2114 residues)

8snx

Cryo-em structure of the respiratory syncytial virus polymerase (l:p) bound to the leader promoter

Source: Respiratory syncytial virus a2. Organism_taxid: 1972429. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Organism_taxid: 1972429

Chain: A (1386 residues)

8sny

Cryo-em structure of the respiratory syncytial virus polymerase (l:p) bound to the trailer complementary promoter

Source: Respiratory syncytial virus a2. Organism_taxid: 1972429. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Organism_taxid: 1972429

Chain: A (1388 residues)

8tw4

Tcr in nanodisc nd-i

Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: homo sapiens. Expression_system_taxid: 9606. Homo sapiens, escherichia coli str. K-12 substr. Mg1655. Organism_taxid: 9606, 511145. Gene: trbv6-5, trb, mcherry.

Chains: X, Y (17 residues)

8v38

Structure of the human systemic rnai defective transmembrane protein 1 (hsidt1)

Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: sidt1. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293s gnti-. Expression_system_atcc_number: crl-3022.

Chains: A, B (618 residues)

8x01

Structure of the mumps virus l protein (state2) bound by phosphoprotein tetramer

Source: Mumps orthorubulavirus. Organism_taxid: 2560602. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Strain: jeryl-lynn. Expression_system_taxid: 7108

Chain: A (1350 residues)

8yxl

Structure of c-terminal domain of l protein from mumps virus

Source: Mumps orthorubulavirus. Organism_taxid: 2560602. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Chain: A (699 residues)

8yxm

Structure of n-terminal domain of l protein bound with phosphoprotein from mumps virus

Source: Mumps orthorubulavirus. Organism_taxid: 2560602. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chain: A (1368 residues)

8yxp

Structure of mumps virus l protein (state2)

Source: Mumps orthorubulavirus. Organism_taxid: 2560602. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Chain: A (1350 residues)

8zpv

Nipah virus polymerase complex

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v/c. Expression_system_taxid: 7108

Chain: A (1275 residues)

9bdq

The structure of niv l-p complex

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v/w/c. Expression_system_taxid: 7108

Chain: A (1301 residues)

9cgi

Cryo-em structure of the nipah virus polymerase (l) protein in complex with the tetrameric phosphoprotein (p)

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: p/v/c.

Chain: A (1314 residues)

9cok

Cryo-em structure of the nipah virus (malaysia strain) l:p complex

Source: Henipavirus nipahense. Organism_taxid: 3052225. Gene: p/v/c. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chain: A (1241 residues)

9cwo

Cryo em structure of nipah virus l-p polymerase complex

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: baculovirus expression vector pfastbac1-hm. Expression_system_taxid: 274590. Gene: p/v/w/c. Expression_system_taxid: 274590

Chain: A (1193 residues)

9dus

Cryo-em structure of the measles virus polymerase (l) protein in complex with the tetrameric phosphoprotein (p)

Source: Measles virus strain edmonston-b. Organism_taxid: 70146. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v. Expression_system_taxid: 7108

Chain: A (1253 residues)

9dut

Cryo-em structure of the measles virus polymerase (l) protein in complex with the tetrameric phosphoprotein (p) and c protein

Source: Measles virus strain edmonston-b. Organism_taxid: 70146. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v. Gene: c, p. Expression_system_taxid: 7108

Chain: A (1946 residues)

9ftf

Crystal structure of the connecting domain of the nipah virus large protein

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Chain: A (260 residues)

9fux

Cryo-em structure of the nipah virus polymerase (l) bound to the tetrameric phosphoprotein (p)

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v/c. Expression_system_taxid: 7108

Chain: A (1286 residues)

9gjt

Structure of nipah virus RNA polymerase complex - apo state

Source: Henipavirus nipahense. Organism_taxid: 3052225. Gene: p/v/c. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_taxid: 7111

Chain: A (1233 residues)

9gju

Structure of replicating nipah virus RNA polymerase complex - RNA- bound state

Source: Henipavirus nipahense. Organism_taxid: 3052225. Gene: p/v/c. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Synthetic: yes. Expression_system_taxid: 7111

Chain: A (2020 residues)

Bound ligand: Het Group GNP is 45.00% similar to enzyme product S-adenosyl-L- homocysteine

9ip2

Cryo-em structure of the RNA-dependent RNA polymerase complex from marburg virus

Source: Marburg virus - musoke, kenya, 1980, escherichia coli k-12. Organism_taxid: 33727, 83333. Gene: male, b4034, jw3994. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Escherichia coli k-12, marburg virus - musoke, kenya, 1980. Organism_taxid: 83333, 33727.

Chain: A (1363 residues)

9ip3

Cryo-em structure of the RNA-dependent RNA polymerase complex in a compact conformation from ebola virus

Source: Escherichia coli (strain k12), ebola virus - eckron (zaire, 1976). Organism_taxid: 83333, 129000. Gene: male, b4034, jw3994. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: male, b4034, jw3994, vp35. Expression_system_taxid: 7108

Chain: A (1365 residues)

9ip4

Cryo-em structure of the RNA-dependent RNA polymerase complex from marburg virus

Source: Marburg virus - musoke, kenya, 1980, escherichia coli k-12. Organism_taxid: 33727, 83333. Gene: male, b4034, jw3994. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Escherichia coli k-12, marburg virus - musoke, kenya, 1980. Organism_taxid: 83333, 33727.

Chain: A (1364 residues)

9ir3

Cryo-em structure of nipah virus l-p polymerase complex

Source: Nipah virus. Organism_taxid: 3052225. Gene: l. Expressed in: spodoptera. Expression_system_taxid: 7106. Gene: p/v/c. Expression_system_taxid: 7106

Chain: A (1253 residues)

9ir4

Cryo-em structure of nipah virus l-p (h1165y) polymerase complex

Source: Nipah virus. Organism_taxid: 3052225. Gene: l. Expressed in: spodoptera. Expression_system_taxid: 7106. Gene: p/v/c. Expression_system_taxid: 7106

Chain: A (1260 residues)

9iv9

Cryo-em structure of a truncated nipah virus l protein bound by phosphoprotein tetramer

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda ascovirus 1c. Expression_system_taxid: 113371. Gene: p/v/c. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Chain: A (1288 residues)

9iva

Cryo-em structure of the full-length nipah virus l protein bound by phosphoprotein tetramer

Source: Henipavirus nipahense. Organism_taxid: 3052225. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v/c. Expression_system_taxid: 7108

Chain: A (1275 residues)

9knq

Measles virus l-p complex in apo state

Source: Measles virus strain ichinose-b95a. Organism_taxid: 645098. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: p/v. Expression_system_taxid: 7111

Chain: A (1284 residues)

9muw

Cryo-em structure of a truncated nipah virus (malaysia strain) l:p complex

Source: Henipavirus nipahense. Organism_taxid: 3052225. Strain: malaysia. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: p/v/c. Expression_system_taxid: 7108

Chain: A (1208 residues)

9mzh

Cryo-em structure of the nipah virus polymerase containing the connecting domain

Source: Henipavirus nipahense. Organism_taxid: 3052225. Gene: p/v/c. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_taxid: 7108

Chains: H, A (121 residues)