PDBsum entry 6y8c

Transferase

PDB id: 6y8c

Contents

Protein chains

339 a.a.

18 a.a.

Ligands

ATP

DMS ×5

MPD ×2

Metals

_MG

Waters ×305

PDB id:

6y8c

Name:

Transferase

Title:

Crystal structure of the camp-dependent protein kinase a cocrystallized with atp and pki (5-24)

Structure:

Camp-dependent protein kinase catalytic subunit alpha. Chain: a. Synonym: pka c-alpha. Engineered: yes. Camp-dependent protein kinase inhibitor alpha. Chain: b. Synonym: pki-alpha,camp-dependent protein kinase inhibitor, muscle/brain isoform. Engineered: yes

Source:

Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Gene: prkaca. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Mus musculus. Mouse.

Resolution:

1.76Å R-factor: 0.162 R-free: 0.190

Authors:

M.Oebbeke,A.Heine,G.Klebe

Key ref:

M.Oebbeke et al. A crystallographic study of camp-Dependent protein ki in complex with different fasudil-Derivatives. To be published, .

Date:

04-Mar-20 Release date: 30-Sep-20

Protein chain

P25321  (KAPCA_CRIGR) -  cAMP-dependent protein kinase catalytic subunit alpha from Cricetulus griseus

Seq: 351 a.a.

Struc: 339 a.a.*

Protein chain

P63248  (IPKA_MOUSE) -  cAMP-dependent protein kinase inhibitor alpha from Mus musculus

Seq: 76 a.a.

Struc: 18 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = ATP) corresponds exactly) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = ATP) corresponds exactly) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site