PDBsum entry 6f3c

DNA

PDB id: 6f3c

Contents

DNA/RNA

Ligands

PT9-PT9

Metals

_MG

Waters ×9

PDB id:

6f3c

Name:

DNA

Title:

The cytotoxic [pt(h2bapbpy)] platinum complex interacting with the cgtacg hexamer

Structure:

DNA (5'-d( Gp Tp Ap Cp G)-3'). Chain: b, d. Engineered: yes. DNA (5'-d( Gp Tp Ap Cp G)-3'). Chain: c, a. Engineered: yes

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

2.30Å R-factor: 0.261 R-free: 0.292

Authors:

M.Ferraroni,C.Bazzicalupi,P.Gratteri,F.Papi

Key ref:

V.H.S.van Rixel et al. (2019). Induction of a Four-Way Junction Structure in the DNA Palindromic Hexanucleotide 5'-d(CGTACG)-3' by a Mononuclear Platinum Complex. Angew Chem Int Ed Engl, 58, 9378-9382. PubMed id: 31046177 DOI: 10.1002/anie.201814532

Date:

28-Nov-17 Release date: 29-May-19

DNA/RNA chains

C-G-T-A-C-G 6 bases

C-G-T-A-C-G 6 bases

G-T-A-C-G 5 bases

G-T-A-C-G 5 bases

DOI no: 10.1002/anie.201814532

Angew Chem Int Ed Engl 58:9378-9382 (2019)

PubMed id: 31046177

Induction of a Four-Way Junction Structure in the DNA Palindromic Hexanucleotide 5'-d(CGTACG)-3' by a Mononuclear Platinum Complex.

V.H.S.van Rixel, A.Busemann, M.F.Wissingh, S.L.Hopkins, B.Siewert, C.van de Griend, M.A.Siegler, T.Marzo, F.Papi, M.Ferraroni, P.Gratteri, C.Bazzicalupi, L.Messori, S.Bonnet.

ABSTRACT

Four-way junctions (4WJs) are supramolecular DNA assemblies comprising four interacting DNA strands that in biology are involved in DNA-damage repair. In this study, a new mononuclear platinum(II) complex 1 was prepared that is capable of driving the crystallization of the DNA oligomer 5'-d(CGTACG)-3' specifically into a 4WJ-like motif. In the crystal structure of the 1-CGTACG adduct, the distorted-square-planar platinum complex binds to the core of the 4WJ-like motif through π-π stacking and hydrogen bonding, without forming any platinum-nitrogen coordination bonds. Our observations suggest that the specific molecular properties of the metal complex are crucially responsible for triggering the selective assembly of this peculiar DNA superstructure.