PDBsum entry 5msh

Virus

PDB id: 5msh

Contents

Protein chains

182 a.a.

369 a.a.

PDB id:

5msh

Name:

Virus

Title:

Cowpea mosaic virus top component (cpmv-t) - naturally occurring empty particles

Structure:

Cowpea mosaic virus small subunit. Chain: a. Synonym: genome polyprotein m,p2. Engineered: yes. Cowpea mosaic virus large subunit. Chain: b. Synonym: genome polyprotein m,p2. Engineered: yes

Source:

Cowpea mosaic virus. Cpmv. Organism_taxid: 12264. Strain: sb. Expressed in: nicotiana benthamiana. Expression_system_taxid: 4100. Expression_system_taxid: 4100

Authors:

E.L.Hesketh,Y.Meshcheriakova,R.F.Thompson,G.P.Lomonossoff,N.A.Ranson

Key ref:

E.L.Hesketh et al. (2017). The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Sci Rep, 7, 539. PubMed id: 28373698

Date:

04-Jan-17 Release date: 19-Jul-17

Protein chain

P03599  (POL2_CPMVS) -  RNA2 polyprotein from Cowpea mosaic virus (strain SB)

Seq: 1046 a.a.

Struc: 182 a.a.

Protein chain

P03599  (POL2_CPMVS) -  RNA2 polyprotein from Cowpea mosaic virus (strain SB)

Seq: 1046 a.a.

Struc: 369 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?

Sci Rep 7:539 (2017)

PubMed id: 28373698

The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy.

E.L.Hesketh, Y.Meshcheriakova, R.F.Thompson, G.P.Lomonossoff, N.A.Ranson.

ABSTRACT

Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.