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PDBsum entry 5msh
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References listed in PDB file
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Key reference
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Title
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The structures of a naturally empty cowpea mosaic virus particle and its genome-Containing counterpart by cryo-Electron microscopy.
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Authors
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E.L.Hesketh,
Y.Meshcheriakova,
R.F.Thompson,
G.P.Lomonossoff,
N.A.Ranson.
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Ref.
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Sci Rep, 2017,
7,
539.
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PubMed id
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Abstract
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Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an
intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in
biotechnology applications such as nanotechnology. Here, we report high
resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing
RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these
structures is sufficient to visualise large amino acids. We have refined an
atomic model for each map and identified an essential amino acid involved in
genome encapsidation. This work has furthered our knowledge of Picornavirales
genome encapsidation and will assist further work in the development of CPMV as
a biotechnological tool.
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