PDBsum entry 5m0c

Transferase

PDB id: 5m0c

Contents

Protein chains

338 a.a.

19 a.a.

Ligands

7CB

MRD

DMS

Waters ×301

PDB id:

5m0c

Name:

Transferase

Title:

Cocrystal structure of camp-dependent protein kinase (pka) in complex with the fasudil-fragment isoquinoline-5-sulfonamide

Structure:

Camp-dependent protein kinase catalytic subunit alpha. Chain: a. Synonym: pka c-alpha. Engineered: yes. Other_details: phosphorylated at ser11, thr198, ser339, the first 15 residues are not well defined in the electron density.. Camp-dependent protein kinase inhibitor alpha. Chain: b. Synonym: pki-alpha,camp-dependent protein kinase inhibitor,

Source:

Cricetulus griseus. Chinese hamster. Organism_taxid: 10029. Gene: prkaca. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Homo sapiens. Human.

Resolution:

1.73Å R-factor: 0.174 R-free: 0.200

Authors:

B.Wienen-Schmidt,A.Heine,G.Klebe

Key ref:

B.Wienen-Schmidt et al. Cocrystal structure of camp-Dependent protein kinase in complex with different fasudil-Derivatives. To be published, .

Date:

04-Oct-16 Release date: 06-Jun-18

Protein chain

P25321  (KAPCA_CRIGR) -  cAMP-dependent protein kinase catalytic subunit alpha from Cricetulus griseus

Seq: 351 a.a.

Struc: 338 a.a.*

Protein chain

P61925  (IPKA_HUMAN) -  cAMP-dependent protein kinase inhibitor alpha from Homo sapiens

Seq: 76 a.a.

Struc: 19 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site