PDBsum entry 5ikc

Transferase

PDB id: 5ikc

Contents

Protein chains

213 a.a.

211 a.a.

89 a.a.

Metals

_CL ×2

Waters ×385

PDB id:

5ikc

Name:

Transferase

Title:

X-ray structure of the n-terminal domain of human doublecortin in complex with fab

Structure:

Mab 6h10 light chain. Chain: a, l. Engineered: yes. Ighg protein. Chain: b, h. Engineered: yes. Neuronal migration protein doublecortin. Chain: m, n. Fragment: n-terminal domain, unp residues 133-221.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: lc. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ighg. Homo sapiens. Human.

Resolution:

2.06Å R-factor: 0.201 R-free: 0.265

Authors:

A.Ruf,M.Stihle,J.Benz,R.Thoma,M.G.Rudolph

Key ref:

D.Burger et al. (2016). Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies. J Biol Chem, 291, 16292-16306. PubMed id: 27226599 DOI: 10.1074/jbc.M116.726547

Date:

03-Mar-16 Release date: 18-May-16

Protein chains

A0A0U5BC76  (A0A0U5BC76_MOUSE) -  MAb 6H10 light chain from Mus musculus

Seq: 234 a.a.

Struc: 213 a.a.*

Protein chains

Q569X1  (Q569X1_MOUSE) -  Ighg protein from Mus musculus

Seq: 476 a.a.

Struc: 211 a.a.*

Protein chains

O43602  (DCX_HUMAN) -  Neuronal migration protein doublecortin from Homo sapiens

Seq: 365 a.a.

Struc: 89 a.a.*

* PDB and UniProt seqs differ at 57 residue positions (black crosses)

DOI no: 10.1074/jbc.M116.726547

J Biol Chem 291:16292-16306 (2016)

PubMed id: 27226599

Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.

D.Burger, M.Stihle, A.Sharma, P.Di Lello, J.Benz, B.D'Arcy, M.Debulpaep, D.Fry, W.Huber, T.Kremer, T.Laeremans, H.Matile, A.Ross, A.C.Rufer, G.Schoch, M.O.Steinmetz, J.Steyaert, M.G.Rudolph, R.Thoma, A.Ruf.

ABSTRACT

No abstract given.