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PDBsum entry 5i8h
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Immune system
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PDB id
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5i8h
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Contents |
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455 a.a.
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132 a.a.
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210 a.a.
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223 a.a.
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212 a.a.
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228 a.a.
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PDB id:
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| Name: |
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Immune system
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Title:
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Crystal structure of HIV-1 bg505 sosip.664 prefusion env trimer in complex with v3 loop-targeting antibody pgt122 fab and fusion peptide-targeting antibody vrc34.01 fab
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Structure:
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Bg505 sosip.664 gp120. Chain: a, c. Synonym: endogenous retrovirus group k member 113 env polyprotein, endogenous retrovirus group k member 13-1 env polyprotein,endogenous retrovirus group k member 18 env polyprotein,endogenous retrovirus group k member 19 env polyprotein,endogenous retrovirus group k member 21 env polyprotein,endogenous retrovirus group k member 24 env polyprotein,endogenous retrovirus group k member 25 env polyprotein, endogenous retrovirus group k member 6 env polyprotein,endogenous
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Source:
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Human immunodeficiency virus 1. Organism_taxid: 11676. Gene: env. Expressed in: homo sapiens. Expression_system_taxid: 9606. Homo sapiens. Organism_taxid: 9606. Expression_system_taxid: 9606
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Resolution:
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4.30Å
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R-factor:
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0.283
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R-free:
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0.309
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Authors:
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K.Xu,T.Zhou,P.D.Kwong
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Key ref:
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R.Kong
et al.
(2016).
Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody.
Science,
352,
828-833.
PubMed id:
DOI:
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Date:
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18-Feb-16
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Release date:
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25-May-16
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PROCHECK
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Headers
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References
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Q2N0S6
(Q2N0S6_HV1) -
Envelope glycoprotein gp160 from Human immunodeficiency virus type 1
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Seq:
Struc:
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860 a.a.
455 a.a.*
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Q2N0S6
(Q2N0S6_HV1) -
Envelope glycoprotein gp160 from Human immunodeficiency virus type 1
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Seq:
Struc:
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860 a.a.
132 a.a.*
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No UniProt id for this chain
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No UniProt id for this chain
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DOI no:
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Science
352:828-833
(2016)
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PubMed id:
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Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody.
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R.Kong,
K.Xu,
T.Zhou,
P.Acharya,
T.Lemmin,
K.Liu,
G.Ozorowski,
C.Soto,
J.D.Taft,
R.T.Bailer,
E.M.Cale,
L.Chen,
C.W.Choi,
G.Y.Chuang,
N.A.Doria-Rose,
A.Druz,
I.S.Georgiev,
J.Gorman,
J.Huang,
M.G.Joyce,
M.K.Louder,
X.Ma,
K.McKee,
S.O'Dell,
M.Pancera,
Y.Yang,
S.C.Blanchard,
W.Mothes,
D.R.Burton,
W.C.Koff,
M.Connors,
A.B.Ward,
P.D.Kwong,
J.R.Mascola.
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ABSTRACT
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The HIV-1 fusion peptide, comprising 15 to 20 hydrophobic residues at the N
terminus of the Env-gp41 subunit, is a critical component of the virus-cell
entry machinery. Here, we report the identification of a neutralizing antibody,
N123-VRC34.01, which targets the fusion peptide and blocks viral entry by
inhibiting conformational changes in gp120 and gp41 subunits of Env required for
entry. Crystal structures of N123-VRC34.01 liganded to the fusion peptide, and
to the full Env trimer, revealed an epitope consisting of the N-terminal eight
residues of the gp41 fusion peptide and glycan N88 of gp120, and molecular
dynamics showed that the N-terminal portion of the fusion peptide can be
solvent-exposed. These results reveal the fusion peptide to be a neutralizing
antibody epitope and thus a target for vaccine design.
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