PDBsum entry 4z15

Isomerase/isomerase inhibitor

PDB id: 4z15

Contents

Protein chains

114 a.a.

Ligands

IPA

SO4 ×3

Waters ×276

PDB id:

4z15

Name:

Isomerase/isomerase inhibitor

Title:

Mif in complex with 3-(2-furylmethyl)-2-thioxo-1,3-thiazolan-4-one

Structure:

Macrophage migration inhibitory factor. Chain: a, b, c. Synonym: mif,glycosylation-inhibiting factor,gif,l-dopachrome isomerase,l-dopachrome tautomerase,phenylpyruvate tautomerase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mif, glif, mmif. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.60Å R-factor: 0.180 R-free: 0.210

Authors:

T.Y.Cho

Key ref:

A.K.Singh et al. (2017). Structural basis for decreased induction of class IB PI3-kinases expression by MIF inhibitors. J Cell Mol Med, 21, 142-153. PubMed id: 27619729 DOI: 10.1111/jcmm.12949

Date:

26-Mar-15 Release date: 28-Sep-16

Protein chains

P14174  (MIF_HUMAN) -  Macrophage migration inhibitory factor from Homo sapiens

Seq: 115 a.a.

Struc: 114 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.5.3.2.1 - phenylpyruvate tautomerase.
• Reaction: 3-phenylpyruvate = enol-phenylpyruvate
• Enzyme class 3: E.C.5.3.3.12 - L-dopachrome isomerase.

Pathway:

• Reaction: L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
• Cofactor: Zn(2+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1111/jcmm.12949

J Cell Mol Med 21:142-153 (2017)

PubMed id: 27619729

Structural basis for decreased induction of class IB PI3-kinases expression by MIF inhibitors.

A.K.Singh, G.Pantouris, S.Borosch, S.Rojanasthien, T.Y.Cho.

ABSTRACT

No abstract given.