PDBsum entry 4xk8

Photosynthesis

PDB id: 4xk8

Contents

Protein chains

742 a.a.

733 a.a.

80 a.a.

141 a.a.

63 a.a.

151 a.a.

95 a.a.

90 a.a.

29 a.a.

39 a.a.

45 a.a.

153 a.a.

195 a.a.

206 a.a.

218 a.a.

196 a.a.

30 a.a.

46 a.a.

Ligands

CLA ×285

PQN ×4

LHG ×11

BCR ×52

SF4 ×6

HTG ×6

LMT

DGD ×2

LMG ×5

CHL ×24

LUT ×10

XAT ×8

Waters ×185

PDB id:

4xk8

Name:

Photosynthesis

Title:

Crystal structure of plant photosystem i-lhci super-complex at 2.8 angstrom resolution

Structure:

Photosystem i p700 chlorophyll a apoprotein a1. Chain: a, a. Synonym: psi-a,psaa. Engineered: yes. Photosystem i p700 chlorophyll a apoprotein a2. Chain: b, b. Synonym: psi-b,psab. Engineered: yes. Photosystem i iron-sulfur center.

Source:

Synthetic: yes. Pisum sativum. Garden pea. Organism_taxid: 3888. Organism_taxid: 3888

Resolution:

2.80Å R-factor: 0.212 R-free: 0.248

Authors:

M.Suga,X.Qin,T.Kuang,J.R.Shen

Key ref:

X.Qin et al. (2015). Photosynthesis. Structural basis for energy transfer pathways in the plant PSI-LHCI supercomplex. Science, 348, 989-995. PubMed id: 26023133 DOI: 10.1126/science.aab0214

Date:

10-Jan-15 Release date: 10-Jun-15

Protein chains

P05310  (PSAA_PEA) -  Photosystem I P700 chlorophyll a apoprotein A1 from Pisum sativum

Seq: 758 a.a.

Struc: 742 a.a.*

Protein chains

P05311  (PSAB_PEA) -  Photosystem I P700 chlorophyll a apoprotein A2 from Pisum sativum

Seq: 734 a.a.

Struc: 733 a.a.*

Protein chains

P10793  (PSAC_PEA) -  Photosystem I iron-sulfur center from Pisum sativum

Seq: 81 a.a.

Struc: 80 a.a.

Protein chains

A5Z2K3  (A5Z2K3_SOYBN) -  Photosystem I reaction center subunit II, chloroplastic from Glycine max

Seq: 210 a.a.

Struc: 141 a.a.

Protein chains

E1C9K6  (E1C9K6_PEA) -  Photosystem I reaction center subunit IV from Pisum sativum

Seq: 64 a.a.

Struc: 63 a.a.*

Protein chains

P12355  (PSAF_SPIOL) -  Photosystem I reaction center subunit III, chloroplastic from Spinacia oleracea

Seq: 231 a.a.

Struc: 151 a.a.*

Protein chains

P12357  (PSAG_SPIOL) -  Photosystem I reaction center subunit V, chloroplastic from Spinacia oleracea

Seq: 167 a.a.

Struc: 95 a.a.*

Protein chains

C6T221  (C6T221_SOYBN) -  Photosystem I reaction center subunit VI from Glycine max

Seq: 139 a.a.

Struc: 90 a.a.*

Protein chain

P17227  (PSAI_PEA) -  Photosystem I reaction center subunit VIII from Pisum sativum

Seq: 40 a.a.

Struc: 29 a.a.

Protein chains

A4GGC6  (PSAJ_PHAVU) -  Photosystem I reaction center subunit IX from Phaseolus vulgaris

Seq: 44 a.a.

Struc: 39 a.a.

Protein chain

E1C9L3  (E1C9L3_PEA) -  PSI-K from Pisum sativum

Seq: 84 a.a.

Struc: 45 a.a.

Protein chains

E1C9L1  (E1C9L1_PEA) -  PSI subunit V from Pisum sativum

Seq: 161 a.a.

Struc: 153 a.a.

Protein chains

Q01667  (CAB6_ARATH) -  Chlorophyll a-b binding protein 6, chloroplastic from Arabidopsis thaliana

Seq: 241 a.a.

Struc: 195 a.a.*

Protein chains

Q41038  (Q41038_PEA) -  Chlorophyll a-b binding protein, chloroplastic from Pisum sativum

Seq: 269 a.a.

Struc: 206 a.a.

Protein chains

Q32904  (CB23_PEA) -  Chlorophyll a-b binding protein 3, chloroplastic from Pisum sativum

Seq: 275 a.a.

Struc: 218 a.a.

Protein chains

Q9SQL2  (CB24_PEA) -  Chlorophyll a-b binding protein P4, chloroplastic from Pisum sativum

Seq: 252 a.a.

Struc: 196 a.a.*

Protein chain

P17227  (PSAI_PEA) -  Photosystem I reaction center subunit VIII from Pisum sativum

Seq: 40 a.a.

Struc: 30 a.a.

Protein chain

E1C9L3  (E1C9L3_PEA) -  PSI-K from Pisum sativum

Seq: 84 a.a.

Struc: 46 a.a.

* PDB and UniProt seqs differ at 29 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, a, b, c: E.C.1.97.1.12 - photosystem I.
• Reaction: reduced [plastocyanin] + hnu + oxidized [2Fe-2S]-[ferredoxin] = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin]

DOI no: 10.1126/science.aab0214

Science 348:989-995 (2015)

PubMed id: 26023133

Photosynthesis. Structural basis for energy transfer pathways in the plant PSI-LHCI supercomplex.

X.Qin, M.Suga, T.Kuang, J.R.Shen.

ABSTRACT

Photosynthesis converts solar energy to chemical energy by means of two large pigment-protein complexes: photosystem I (PSI) and photosystem II (PSII). In higher plants, the PSI core is surrounded by a large light-harvesting complex I (LHCI) that captures sunlight and transfers the excitation energy to the core with extremely high efficiency. We report the structure of PSI-LHCI, a 600-kilodalton membrane protein supercomplex, from Pisum sativum (pea) at a resolution of 2.8 angstroms. The structure reveals the detailed arrangement of pigments and other cofactors—especially within LHCI—as well as numerous specific interactions between the PSI core and LHCI. These results provide a firm structural basis for our understanding on the energy transfer and photoprotection mechanisms within the PSI-LHCI supercomplex.