PDBsum entry 4fbk

Hydrolase, protein binding

PDB id: 4fbk

Contents

Protein chains

424 a.a.

Ligands

SO4 ×4

Metals

_MN ×2

Waters ×210

PDB id:

4fbk

Name:

Hydrolase, protein binding

Title:

Crystal structure of a covalently fused nbs1-mre11 complex with one manganese ion per active site

Structure:

DNA repair and telomere maintenance protein nbs1,DNA repair protein rad32 chimeric protein. Chain: a, b. Fragment: unp o43070 residues 474-531, unp q09683 residues 15-413. Engineered: yes

Source:

Schizosaccharomyces pombe. Organism_taxid: 4896. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.38Å R-factor: 0.223 R-free: 0.242

Authors:

C.B.Schiller,K.Lammens,K.P.Hopfner

Key ref:

C.B.Schiller et al. (2012). Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling. Nat Struct Biol, 19, 693-700. PubMed id: 22705791

Date:

23-May-12 Release date: 20-Jun-12

Protein chains

O43070  (NBS1_SCHPO) -  DNA repair and telomere maintenance protein nbs1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 613 a.a.

Struc: 424 a.a.*

Protein chains

Q09683  (RAD32_SCHPO) -  Double-strand break repair protein rad32 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 649 a.a.

Struc: 424 a.a.*

* PDB and UniProt seqs differ at 113 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.-.-

Nat Struct Biol 19:693-700 (2012)

PubMed id: 22705791

Structure of Mre11-Nbs1 complex yields insights into ataxia-telangiectasia-like disease mutations and DNA damage signaling.

C.B.Schiller, K.Lammens, I.Guerini, B.Coordes, H.Feldmann, F.Schlauderer, C.Möckel, A.Schele, K.Strässer, S.P.Jackson, K.P.Hopfner.

ABSTRACT

No abstract given.