PDBsum entry 4f1h

Hydrolase/DNA

PDB id: 4f1h

Contents

Protein chains

250 a.a.

DNA/RNA

Ligands

PO4

GOL ×4

Metals

_MG

Waters ×596

PDB id:

4f1h

Name:

Hydrolase/DNA

Title:

Crystal structure of tdp2 from danio rerio complexed with a single strand DNA

Structure:

DNA (5'-d(p Tp Gp Cp Ap G)-3'). Chain: c. Engineered: yes. Tyrosyl-DNA phosphodiesterase 2. Chain: a. Synonym: tyr-DNA phosphodiesterase 2, 5'-tyrosyl-DNA phosphodiesterase, 5'-tyr-DNA phosphodiesterase, traf and tnf receptor-associated protein homolog. Engineered: yes.

Source:

Synthetic: yes. Danio rerio. Leopard danio,zebra danio,zebra fish. Organism_taxid: 7955. Gene: tdp2, ttrap, ttrapl, si:ch211-81e5.5, si:dkey-218n20.5. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

1.66Å R-factor: 0.164 R-free: 0.193

Authors:

K.Shi,K.Kurahashi,H.Aihara

Key ref:

K.Shi et al. (2012). Structural basis for recognition of 5'-phosphotyrosine adducts by Tdp2. Nat Struct Biol, 19, 1372-1377. PubMed id: 23104058

Date:

06-May-12 Release date: 31-Oct-12

Protein chains

Q5XJA0  (TYDP2_DANRE) -  Tyrosyl-DNA phosphodiesterase 2 from Danio rerio

Seq: 369 a.a.

Struc: 250 a.a.

DNA/RNA chain

T-G-C-A-G 5 bases

Enzyme reactions

• Enzyme class: E.C.3.1.4.- - ?????

Nat Struct Biol 19:1372-1377 (2012)

PubMed id: 23104058

Structural basis for recognition of 5'-phosphotyrosine adducts by Tdp2.

K.Shi, K.Kurahashi, R.Gao, S.E.Tsutakawa, J.A.Tainer, Y.Pommier, H.Aihara.

ABSTRACT

No abstract given.